| 糖皮质激素对糖皮质激素受体的调节 Ⅰ.人体肝癌细胞系(SMMC-7721)的研究 |
| |
| 引用本文： | 孙尔维,卢建,徐仁宝,董荣春. 糖皮质激素对糖皮质激素受体的调节 Ⅰ.人体肝癌细胞系(SMMC-7721)的研究[J]. 第二军医大学学报, 1989, 0(1) |
| |
| 作者姓名： | 孙尔维 卢建 徐仁宝 董荣春 |
| |
| 作者单位： | 第二军医大学病理生理学教研室(孙尔维，卢建，徐仁宝)，第二军医大学病理解剖学教研室(董荣春) |
| |
| 摘 要： | 建立了糖皮质激素受体(GR)的计数瓶培养测定法。用此法研究了10~(-8)、10~(-7)和10~(-6)mol/L地塞米松对人体肝癌细胞系(SMMC-7721)GR的影响,发现地塞米松使GR减少。去除地塞米松后24h,GR恢复到接近原来的水平。结果证明,糖皮质激素对GR呈现负调节。
|
| 关 键 词： | 糖皮质激素 糖皮质激素受体 受体负调节 人体肝癌细胞系 地塞米松 |
REGULATION OF GLUCOCORTICOID RECEPTOR BY GLUCOCORTICOIDSs STUDY ON THE HUMAN HEPATOCELLULAR CARCINOMA CELL LINE (SMMC-7721) |
| |
| Sun Erwei,et al. REGULATION OF GLUCOCORTICOID RECEPTOR BY GLUCOCORTICOIDSs STUDY ON THE HUMAN HEPATOCELLULAR CARCINOMA CELL LINE (SMMC-7721)[J]. Former Academic Journal of Second Military Medical University, 1989, 0(1) |
| |
| Authors: | Sun Erwei et al |
| |
| Affiliation: | Department of Pathophysiology |
| |
| Abstract: | The effects of dexamethasone(Dex)on the 3H-Dex specific binding were studied in the human heptatocellular carcinoma cell line(SMMC-7721) by using counting vial culture assay.Cells were cultivated in the counting vials, as th; grew well and the monolayer cells adhered to the wall of vials.The cells were cultivated in the mdium containing 10-3,10-7, and 10-6mol/L Dex for 6, 12, 24 and 48 h, and then the specific binding of 3H-Dex at 30nmol/L of the ligand was determined after elimination of the effect of the occupancy of GR.It was found that 3H-Dex specific binding was not changed within 6 h, but decreased to 70% at 12 h and maintained at this level from 12 to 48 h in the Dex pretreated cells.The specific binding was recovered approximately to the control level 24 h after removal of Dex.These results indicate that glucocorticoids may down-regulate glucocorticoid receptor in vitro. |
| |
| Keywords: | glucocorticoids glucocorticoid receptor down-regulation of receptor human hepatocellular carcinoma cell dexamethasone |
| 本文献已被 CNKI 等数据库收录！ |
