奉贤地区HBsAg阴性的HBV自然感染母亲对新生儿影响的研究

目的　为揭示HBsAg阴性的乙型肝炎病毒 (HBV)自然感染孕妇的宫内感染及其危险因素。方法　采用多聚酶链反应 (PCR)技术结合酶联免疫吸附法 (ELISA) ,对奉贤地区 131例HBsAg阴性的HBV自然感染孕妇外周血 ,及其分娩后的脐带血进行HBV血清学标志物 (HBVM)和HBVDNA检测。结果　HBsAg阴性的HBV自然感染孕妇宫内的感染率 (除外单一抗 -HBs阳性 )为 5 2 6 7% ;脐血中不同HBVM组合的HBVDNA检出率依次为 :抗 -HBe( )、抗 -HBc( ) >抗 -HBs( )、抗 -HBe( )、抗 -HBc( ) >抗 -HBs( )、抗 -HBe( ) >抗 -HBs( )、抗 -HBc( ) >抗 -HBs( ) ;脐血HBVDNA总检出率为 16 79%。结论　HBsAg阴性的HBV自然感染孕妇也可能发生宫内感染。提议HBsAg阴性的HBV自然感染孕妇和新生儿有进行自动和被动免疫接种的必要性

A study on the influence of HBsAg negative HBV infected pregnant women on newborns

Objective:To go further into the hepatitis B virus(HBV) intrauterine infection and its risk factors in HBsAg negative pregnant women.Method:HBV serum markers and HBV DNA were detected by using enzyme linked immunosorbent assay (ELISA)and polymerase chain reactin (PCR)in 131 HBsAg negative HBV infected pregnant women-induced cord blood of foctuses.Results:The intrauterine infection rates of these women were 52.67% except anti-HBs positive.The positive rates of HBV DNA in cood blood were anti-HBe( ),anti HBc( )>anti-HBs, anti-HBe( ), anti HBc( )>anti-HBs,anti-HBe( )>anti-HBs,anti HBc( )>anti-HBs.The positive rates of HBV DNA in cood blood were 16.79%. Conclusion:The HBV intrauterine infection rates is rather high.HBsAg negative pregnant women may also transmit HBV intrauterine infection.So we suggest that HBsAg negative pregnant women and newborns should be given vaccination against hepatitis B virus and need for abooster dose.