膀胱癌错配修复基因hMSH2表达的研究

目的 探讨错配修复基因hMSH2在膀胱移行细胞癌中的表达情况及其与肿瘤细胞增殖、凋亡的关系.方法 采用免疫组化法检测101例膀胱移行细胞癌错配修复基因hMSH2以及细胞增殖抗原Ki-67的表达情况,原位末端标记(TUNEL)法检测肿瘤细胞凋亡情况.结果 hMSH2表达于非膀胱肿瘤尿路上皮及部分膀胱移行细胞癌的细胞核,膀胱移行细胞癌组低表达率较非膀胱肿瘤膀胱组织组高(P=0.004,＜0.05);膀胱移行细胞癌pT2-pT4组中hMSH2的低表达率比pTis-pT1组的低表达率高(P=0.016,＜0.05);G1、G2、G3三组间低表达率的总体差别有统计学意义(P=0.033,＜0.05),G1+G2组与G3组之间差别有统计学意义(P=0.036＜0.05);有无淋巴结转移组差别无统计学意义(P=0.317).hMSH2弱表达组与强表达组间凋亡指数(AI)均值差异非常显著(P＜0.01),Ki-67标记指数(Ki-67 LI)均值差异不显著(P＞0.05),AI/KI值差异经统计学分析具有统计学意义(P＜0.05).结论 hMSH2基因突变或功能缺失与膀胱移行细胞癌的发生有关,可能系肿瘤发生过程中的重要事件,并可能与肿瘤细胞凋亡有关,而与增殖活性无关.

Study of Expression of hMSH2 in Bladder Transitional Cell Carcinoma

Objeetives To investigate the expression patterns of hMSH2 in human bladder transitional cell cercinoma and to assess the correlation of hMSH2 Drotein to tumor cell prolfferation and apoptosiB.Methods We examined the expression patterns of hMSH2 and Ki-67 protein in 101 patients with transitional cell carcinoma (TCC)of the urinary bladder by immunohistochemical technique,futhermore,we also detected apoptosis rate of TCC by in situ end labeling techinque(TUNEL).Results:Urothelium are demostrated uniform expressin of hMSH2,which was restricted to the crypt nuclei.Results Expression was associated with the least differentiated cancer(P ＜0.05).Expression of hMSH2 was significant lyhigher for pT2-4 tumor than pTis-1(P=0.016,＜0.05),The diffirence expression rate of hMSH2 among GI,G2 and G3 of TCC possessed statistics significance(P=0.033,＜0.05),and a difference of statistical significance(P=0.036.＜0.05)also found between G1+G2 and G3 groups of TCC.while we didn't find any relationship between hMSH2 expression and lymphatic invasion(P=0.317).The apoptisis index of the preserved hMSH2 exprssion groups of TCC was higher compared with reduced expression groups (p＜0.01),and the KI-67LI of reduced hMSH2 expression groupWas slighfly lower than the preserved hMSH2 expression groups,which have no statistic significance diference.the AI/KI of reduced hMSH2 expression groups was lower than preserved hMSH2 groups,and statistic significance diferance was found between them.Conclusions Least differentiated and most invasive tumors are mre likely to have reduced hMSH2 expression.which may centribu in the development of a subset of TCCs owning to genetic mutation.hMSH2 germ were related with the automatic apoptosis of TCC,but no statistically significant was found between hMSH2 emutation and the poliferation activity(Ki67 LI).