双抗体夹心斑点金免疫渗滤法检测血吸虫循环抗原的现场应用

目的 探讨双抗体夹心斑点金免疫渗滤法（S－DIGFA）在现场的应用价值。方法 在原已建立的以抗26kDaGST基因重组蛋白多克隆抗体为捕获抗体兼测示抗体的S－DIGFA的基础上，应用该法检测血吸虫病中度流行区和传播阻断地区人群的血清共1388份，并以双抗体夹心ELISA（S－ELISA）作对照。结果 用S－DIGFA和S－ELISA平行检测血吸虫病中度流行区人群血清300份，阳性检出率分别为15．7％和17．3％；两法检测血吸虫病传播阻断地区人群血清1088份，阳性检出率分别为3．9％和3．7％。结论 S－DIGFA检测循环抗原可在现场扩大和作血清流行病学调查。

FIELD APPLICATION OF DETECTING SCHISTOSOMA CIRCULATING ANTIGEN BY DOUBLE ANTIBODY SANDWICH DOT IMMUNO-GOLD FILTRATION ASSAY

Objective To explore the application value of double antibody sandwich dot immuno gold filtration assay (S DIGFA) in the field. Methods On the basis of developed S DIGFA which used anti 26 kDa GST recombinant protein polyclonal antibody as capturing and detecting antibody, 1388 sera from the population of schistosomiasis middle epidemic area and transmitting blocking area were detected by S DIGFA, and by the double antibody sandwich ELISA(S ELISA) as control. Results Three hundred sera from the population of schistisomiasis middle epidemic area were parallelly detected by S DIGFA and S ELISA. The positive rates were 15 7% and 17 3%, respectively Detecting 1088 sera from the population of transmitting blocking area, the positive rates were 3 9% and 3.7%, respectively. Conclusion The detection of circulating antigen with S DIGFA can be applied in the field, and used as an epidemiological tool for survey.