Lokale pO2- und pH2-Messungen mit Nadelelektroden zum Studium der Sauerstoffversorgung und MikroZirkulation des Innenohres

Summary The local partial pressure of oxygen (pO₂) and the rate of hydrogen elimination were measured in the three scalae of the basal turn of 28 Guinea-pig cochleae under conditions of normoxia, hyperoxia and hypercapnia and with acoustical stimulation with the needle electrodes developed by Baumgärtl and Lübbers.In the scala tympani a pO₂ decrease from the round window toward Corti's organ was registered and pO₂ values of over 100 mm Hg were measured near the membrane of the round window and of 10–40 mm Hg near the basilar membrane depending on how deeply the electrode penetrated into the scala tympani. The pO₂ profiles were changed or reversed when the animal breathed a mixture of 95% oxygen and 5% carbon dioxide and when the round window membrane was covered with agar-agar or paraffine and exteriorly flooded with nitrogen.Acoustical stimulation with a white noise of 85 dB caused a considerable pO₂ drop in the perilymph of the scala tympani while in the endolymph of the scala media we observe only a slight decrease. Intravenous application of dextran of low viscosity leads to a pO₂ increase when the original oxygen value in the scala tympani was low. The half-life of hydrogen in the scala tympani amounts to about 4 min.The results permit the conclusion that, in the area of the cochlear basis, Corti's organ receives its oxygen supply via the capillary system as well as via the membrane of the round window.

Für die Herstellung der Mikroelektroden danken wir Frau G. Malinkewitz und Herrn R. König. Frau M. Goden sind wir für die sorgfältige Hilfe bei der Durchführung der Experimente zu Dank verpflichtet. Ferner gilt unser Dank Herrn Dr. rer. nat. G. Esser für die wertvolle Unterstützung bei den Beschallungsexperimenten.