冲击波对豚鼠螺旋神经节细胞P物质免疫反应的影响

Efiects of blast wave on SP-IR in spiral ganglion cells

Fifteen guinea pigs were exposed to the blast wave of one shot of a 152 mm cannon with a peak pressure value of 178.5 dB (SPL). It was shown by an immunohistochemical method and an image analyzer that on the first day of the exposure, the optical density of the SP IR products was higher in the SG cells than that in the controls, and that it was lower on the third day (P<0.01 ). The optical density of the SP IR products become nor mal compared with that of the controls on the 7th day. No obvious differences could be found among the apical, middle, upper base and base. On the 1st day after the exposure, the SP IR products were found obviously increased in cytoplasm by immunoelectron microscopy. A lot of stained small clear vesicles (50~70 nm) existed in cytoplasm. Some of them formed a round structure around the mitochondrion. The ABR threshold of guinea pigs in different time groups was related to the changes of SP IR in SG cells (r=0.725 1, P<0.05). This suggests that the auditory damage induced by blast waves was correlated obviously to the changes of SP-IR in SG cells.