外科手术切口感染的病原菌耐药性调查

目的探讨外科手术切口术后感染的病原菌及其耐药性。方法采集1999～2003年感染伤口分泌物进行细菌培养，分离致病菌并用常规方法进行鉴定；用琼脂扩散法做药物敏感试验，对致病菌耐药率进行分析。结果4年来我院切口感染标本中共培养出86株病原菌，细菌分布依次为金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌等；菌株中出现耐甲氧西林金黄色葡萄球菌(MRSA)7株，耐甲氧西林凝固酶阴性葡萄球菌(MRCNS)4株，产超广谱β-内酰胺酶(ESBLs)菌7株；经药物敏感试验测定发现以上耐药菌株均显示了严重的多重耐药性；目前，万古霉素和亚胺培南仍具有良好的抗菌作用。结论外科切口术后感染问题值得高度重视，对耐药菌株应严密监测与控制。

Surgical Incision Infection:An Investigation on Bacterial Resistance

OBJECTIVE To study the resistance status of pathogens with surgical incision infection. METHODS The secretion of infected wounds was cultured to detect pathogens which were confirmed by routine methods. The resistant rates were analyzed by adopting disk diffusion test. RESULTS A total of 86 pathogens strains were cultured from the secretion of surgical incision infection in 4 years. Among them,Staphylococcus aureus was the highest,and Pseudomonas aeruginosa and Escherichia coli were followed. Among them 7 strains of meticillin-resistant S. aureus (MRSA),4 strains of meticillin-resistant coagulase negative Staphylococcus (MRCNS) and 7 strains of ESBLs producing bacteria were detected. Almost all of the detected resistant strains showed serious multiple resistance. At present,vancomycin and imipenem still have better activities to the multiple resistant bacteria. CONCLUSIONS Surgical incision infection should be attached importance to. It is time to take some effective measures to supervise and control bacterial resistance.