比索洛尔对β_1肾上腺素受体自身抗体阳性心力衰竭大鼠Bcl-2/Bax的影响

目的探讨比索洛尔在降低β1肾上腺素受体(β1-AR)自身抗体阳性率和抗体滴度的同时,是否可以抑制细胞凋亡。方法将90只SD大鼠中80只采用缩窄腹主动脉方法,建立心力衰竭的大鼠模型,15只死亡,入组65只,将大鼠随机分为治疗组(40只)和非治疗组(25只)。治疗组接受比索洛尔4周治疗后,应用ELISA技术检测两组大鼠血清β1-AR自身抗体阳性率和滴度。应用免疫组织化学测两组大鼠血清β1-AR自身抗体阳性的Bax/Bcl-2。结果治疗组较治疗前β1-AR自身抗体阳性率降低27.5%(P<0.05);非治疗组无统计学差异(P>0.05);治疗组较治疗前β1-AR自身抗体滴度降低(P<0.01);非治疗组β1-AR自身抗体阳性滴度无显著差异(P>0.05);治疗组中Bcl-2的表达在β1-AR自身抗体阳性大鼠比阴性大鼠、非治疗组β1-AR阳性大鼠明显增高(P<0.01);Bax的表达比非治疗组阳性大鼠降低(P<0.01);且比同组阴性略有降低。结论比索洛尔治疗降低心力衰竭大鼠β1-AR自身抗体阳性率及抗体滴度,升高了β1-AR自身抗体阳性心力衰竭大鼠Bcl-2/Bax的比值。

The effects of bisoprolol on Bcl-2/Bax in heart failure rats with positive cardiac β1-AR autoantibodies

Objective To explore whether bisoprolol can inhibit cardiomyocyte apoptosis simultaneously with reduction of the positive rate and antibody titer of β1-AR autoantibodies.Methods Eighty of 90 male SD rats underwent constriction of the abdominal aorta to establish heart failure model.Sixty-five of them were randomly divided into treatment group and without treatment group.ELISA was used to measure β1-AR autoantibody positive rate and average titer.Immunohistochemistry was used to measure Bcl-2/Bax.Results After bisoprolol treatment,β1-AR autoantibody positive rate(P<0.05) was reduced by 27.5% as compared with before treatment,while there was no change in without treatment group(P>0.05).Titer of β1-AR autoantibodies in the treatment group decreased significantly(P<0.01);while there was no significant change in without treatment group(P>0.05).Expression of Bcl-2 in treatment group with positive cardiac β1-AR autoantibodies was significantly increased as compared with that in β1-AR antibody negative and without treatment rats(P<0.01).Expression of Bax in treatment group with positive cardiac β1-AR autoantibodies was depressed significantly as compared with that in without treatment group(P<0.01),and slightly decreased as compared with that in negative group.Conclusions After bisoprolol treatment,β1-AR autoantibodies positive rate and average titer are decreased,and Bcl-2/Bax ratio in heart failure rats with positive cardiac β1-AR autoantibodies is increased.