抑癌基因P53突变与宫颈不典型增生的相关性研究

目的：探讨抑癌基因Ｐ５３突变在宫颈不典型增生形成中的作用和意义。方法：应用聚合酶链反应（ＰＣＲ），单链构象多态性（ＳＳＣＰ）分析法检测了５０例宫颈不典型增生，２６例宫颈息肉，２５例慢性宫颈炎症组织中Ｐ５３外显子７－８的变异。结果：宫颈不典型增生，息肉，炎症组织中均未见Ｐ５３外显子７－８缺失；宫颈不典型增生，息肉组织中ＰＴ５３外显子７－８突变阳性率分别为１６％，３．８５％，二者差异显著；

Study of P53 Gene Mutation in Cervical Atypiccal Hyperplesin

To investigation the role and significane of P53 mutation in the genesis of cervical atypicalhyperplasia.Methods:P53(exon7 - 8 ) mutations were examined in 25 cervical atypical hyperplasia,26 cervical polrpand 25 cervical chronic inflamation by polyrnrase chain reaction (PCR) and single strand confonnaion polymrphism(SSCP). Resulte: The defects of P53 (exon7 - 8)were no foUn, but P53(exon - 8) mutations were detected in 4 of25(16% ) atypical hnperpalsia and in 1 of 26(3. 85% )polyp, and were not found in chronic infalmaion.About P53(exon7-8 ) mutations, there was a apparent difference (P<0.001). between atypical hyperplasia and polyp,but therewas no sighficant difference among light, moderate and geavy degree of atypical hyperplasia (P>0.05). Conclution:P53(exon7-8) mutations in the peis of cervical atypical hyperplasia is a important and early factor.