玻璃体腔注射乳酸环丙沙星对视网膜 影响的研究

目的探讨玻璃体腔注射乳酸环丙沙星治疗方法对视网膜组织的安全性。方法24只兔随机分为4组，每组6只。玻璃体腔内分别注入0.1 ml不同浓度的乳酸环丙沙星，浓度分别为：2 500 、5 000、10 000 μg/ml；对照组注射生理盐水0.1 ml。手术后观察眼底表现，分别行光学显微镜、透射电子显微镜等组织学检查和视网膜电图（ERG）视网膜功能检查。结果250、500 μg组光学显微镜检查结果未见异常，超微结构大致正常；1 000 μg 组光学显微镜下可见视网膜内外核层排列不规则，神经节细胞水肿变性或脱失，透射电子显微镜下可见超微结构改变。视网膜功能检查显示，各组ERG的a～b波波幅注药前后无明显差别。结论玻璃体腔注射国产乳酸环丙沙星安全、抗菌谱广，500 μg以下是较为安全的眼内应用剂量。(中华眼底病杂志,2005,21:180-182)

The effect of intravitreal injection with ciproflaxacin on retina

ObjectiveTo evaluate the security of intravitreal injection with ciproflaxacin to retina.MethodsTweenty-four rabbits were randomly divided into 4 groups with 6 rabbits in each group. 0.1 ml ciproflaxacin in doses of 2 500,5 000,and 10 000 μg was intravitreally injected into the rabbits eyes, retrospectively. And 0.1 ml saline solution was injected into the vitreous body of the rats in the control group. Indirect microscope, light microscope and electroretinogram (ERG) were used to observe the changes of ocular fundus.ResultsNormal results of light microscopy and ultrastructure were found in 250 μg and 500 μg groups; irregularly arranged outer and inner nuclear layers, dropsical or even lost ganglion cells, and ultrastructural changes were in 1 000 μg group. There was no apparent difference of ERG′s a and b amplitudes before and after intravitreal injection with ciproflaxacin in each group.ConclusionIntravitreal injection with ciproflaxacin is safe, and 500 μg or less is the secure dosage in rabbits' eyes. (Chin J Ocul Fundus Dis, 2005,21:180-182)