| 紫杉醇纳米胶束冻干粉针剂的细胞毒性评价 |
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| 引用本文： | 杜卓,余巧,潘昕,钟琳,杨海云,卢建,叶泉英,崔志新.紫杉醇纳米胶束冻干粉针剂的细胞毒性评价[J].现代食品与药品杂志,2014(3):145-148. |
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| 作者姓名： | 杜卓 余巧 潘昕 钟琳 杨海云 卢建 叶泉英 崔志新 |
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| 作者单位： | [1]佛山科学技术学院医学院药学系,广东佛山528000 [2]中山大学药学院药剂学与制药工程学实验室,广东广州510006 [3]惠州卫生职业技术学院药学系,广东惠州516025 [4]南方医科大学第三附属医院,广东广州510630 [5]中山大学孙逸仙纪念医院,广东广州510120 [6]广东省妇幼保健院,广东广州510010 |
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| 基金项目： | 基金项目：广东省自然科学基金（编号：04011632）广东高校优秀青年创新人才培养计划（育苗工程,编号：2012LYM_0130） |
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| 摘 要： | 目的对紫杉醇／聚乙二醇．聚谷氨酸苄酯（PEG-PBLG）纳米胶束冻干粉针剂的体外细胞毒性：进行研究评价。方法采用四噻唑蓝（MTT）法研究高分子嵌段共聚物PEG—PBLG、紫杉醇／PEG—PBLG冻干粉针剂对体外细胞毒性的影响。结果与增溶剂聚氧乙烯蓖麻油的较强细胞毒性相比，高分子材料PEG-PBLG安全性好，仅在200ug/mL浓度时才表现出有细胞毒性；当紫杉醇浓度≤10ug／mL时．紫杉醇／PEG—PBLG冻干粉针剂对2种人癌细胞的毒性均低于相应浓度的市售紫杉醇注射剂（P〈0．01）；结论与市售紫杉醇注射液相比，紫杉醇／PEG-PBLG冻干粉针剂能极大降低药物在体外的细胞毒性，并且该高分子嵌段共聚物本身毒性较低，安全性好，该纳米胶束制剂具有较为广阔的临床应用前景。
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| 关 键 词： | 紫杉醇 聚乙二醇一聚谷氨酸苄酯 嵌段共聚物 纳米胶束 冻干粉针剂 细胞毒性 |
Cell Cytotoxicity Evaluation of PaclitaxeI-Loaded Lyophilized Nano-Micelles |
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| DU Zhuo,YU Qiao,PAN Xin,ZHONG Lin,YANG Hai-yun,LU Jian,YE Quan-ying,CUI Zhi-xin.Cell Cytotoxicity Evaluation of PaclitaxeI-Loaded Lyophilized Nano-Micelles[J].JOurnal of Modern Food and Pharmaceuticals,2014(3):145-148. |
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| Authors: | DU Zhuo YU Qiao PAN Xin ZHONG Lin YANG Hai-yun LU Jian YE Quan-ying CUI Zhi-xin |
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| Institution: | 1. Pharmacy Department, Medical School, Foshan University, Foshan, Guangdong 528000, China; 2. Department of Pharmaceutics, The School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong 510006, China; 3. Pharmacy Department, Huizhou Health Vocational College, Huizhou, Guangdong 516025, China; 4. The Third Affiliate Hospital of Southern Medical University, Guangzhou, Guangdong 510630, China; 5. Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510120, China; 6. Guangdong Women And Children Hospital, Guangzhou, Guangdong 510010, China) |
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| Abstract: | Objective Aim To study the cell cytotoxicity of paclitaxel-loaded lyophihzed nano-micelles （ Polyethylene glycol-polybenzyl-L-glutamate, PEG-PBLG ）in vitro. Methods The cell cytotoxicity of paclitaxel-loaded lyophilized nano-micelles was observed with MTT assay by comparing with the commercially available paclitaxel injections with Cremophor EL. Results The block copolymer PEG-PBLG had little cell cytotoxicity until 200 ug/mL concentration. In low concentration of paclitaxel （ ≤ 10 ug/mL） the paclitaxel-loaded PEG-PBLG lyophilized nano-micelles displayed much less cell cytotoxicity than paclitaxel injections with Cremophor EL （ P 〈 0.01 ）. Conclusion The paclitaxel-loaded PEG-PBLG lyophilized nano-micelles had less cytotoxicity than paclitaxel injections wiLh Cremophor EL. So paclitaxel- loaded PEG-PBLG nano-micelles would be a novel paclitaxel preparation in clinic for the treatment of tumor. |
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| Keywords: | paclitaxel polyethylene glycol-polybenzyl-L-glutamate block copolymer nano-micelle freeze-dried powder for injection cell cytotoxicity |
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