穿心莲内酯对AS早期新西兰兔主动脉LDLR、ABCA1、ApoA1及SRB1表达的影响

目的探讨穿心莲内酯对动脉粥样硬化（As）早期新西兰兔主动脉低密度脂蛋白受体（LDLR）、三磷酸腺苷结合盒转运体（ABCA1）、载脂蛋白A1（ApoA1）以及清道夫受体（SRB1）mRNA表达的影响。方法SPF级新西兰家兔36只，雌雄各半，随机分为6组：空白对照组、模型对照组、非诺贝特组、穿心莲内酯高、中、低3个剂量组，除空白对照组给予普通饲料饲养外，其余各组给予高脂饲料50g，造模喂养4周，4周后按照分组继续进行高脂饲料喂养，各给药组按剂量灌胃给予相应药物。空白对照组、模型对照组同法灌胃给予等体积蒸馏水，连续给药4周。末次给药后1h取主动脉进行LDLR、ABCA1、ApoA1以及sRB1的mRNA表达。结果与模型组比较，穿心莲内酯不同剂量组新西兰兔主动脉LDLR、ABCA1、ApoA1、SRB1 mRNA表达都明显高于模型组，差异有统计学意义（P〈0．05或P〈0．01）。结论穿心莲内酯有明显诱导LDLR、ABCA1、ApoA1、SRB1 mRNA表达的作用。

Effect of Andrographolide on Expression of LDLR,ABCAI, ApoA1, and SRB1 in Early Atherosclerosis Rabbit

Objective To study the effect of andrographolide on the expression of LDLR, ABCA1, ApoA1, and SRB1 in early atherosclerosis rabbit. Methods A total of 36 SPF New Zealand rabbits were chosen and divided into normal control group, model group, fenofibrate group, and High, medium and low dose of andrographolide group. Except for the normal control group, the rest were made hyperlipemia model for 4 weeks. After the modeling, the rabbits were treated with andrographolide for 4 weeks. Results Compared with model group, the expression of LDLR, ABCA1, ApoA1, and SRB1 of the andrographolide group were obviously raised, with the difference was statistically significant （P〈0.05 or P〈 0.01 ）. Conclusion Andrographolide could significantly induced the expression of LDLR, ABCA1, ApoA1, and SRB1 in early atherosclerosis rabbit.