| 高效液相色谱法测定大豆葛根软胶囊中葛根素的含量 |
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| 引用本文： | 方加林,方恒杰.高效液相色谱法测定大豆葛根软胶囊中葛根素的含量[J].今日药学,2014(2):126-128. |
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| 作者姓名： | 方加林 方恒杰 |
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| 作者单位： | [1]揭阳市食品药品检验所,广东揭阳522000 [2]广州白云山制药总厂,广东广州510515 |
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| 摘 要： | 目的建立HPLC法测定保健品大豆葛根软胶囊中葛根素的含量。方法采用AgilentEclipseXDB—C18柱，甲醇-0．1％醋酸水溶液（30：70）为流动相；检测波长为250nm；流速为1．0mL／min；柱温为30℃。结果葛根素在0．0282～0．282mg／mL范围内呈良好的线性关系，r：0．9992，平均回收率99．6％，RSD=1．7％（n=6）。结论本方法灵敏迅速，准确可靠，可用于保健品大豆葛根软胶囊中葛根素的含量测定。
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| 关 键 词： | 葛根素 HPLC 含量测定 保健食品 油脂基质 |
Determination of Puerarin in High Performance Soft Capsules by Reverse-Phase Liquid Chromatography |
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| FANG Jia-lin,FANG Heng-jie.Determination of Puerarin in High Performance Soft Capsules by Reverse-Phase Liquid Chromatography[J].Pharmacy Today,2014(2):126-128. |
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| Authors: | FANG Jia-lin FANG Heng-jie |
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| Institution: | 1. Jieyang Institule for Food and Drug Control, Jieyang, Guangdong 522000, China ; 2. Guangzhou Baiyun Shan Pharmaceutical General Factory, Guangzhou, Guangdong 510515, China) |
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| Abstract: | Objective To establish a new method for determining the content of puerarin by HPLC. Methods Agilent Eclipse XDB-CI8 column was adopted, using methanol- 0. 1% acetate acid solution （30：70）, at detection wavelength of 250 nm. The flow rate was 1.0 mL/min, and column temperature was 30 ℃. Results The linear range of puerarin concentration was 0.028 2 -0. 282 mg/mL, r =0. 999 2. The average recovery of sample was 99.6%, RSD = 1.7% （n = 6 ）. Conclusion This method is sensitive and accurate with good repeatability. It is suitable for the determination of puerarin. |
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| Keywords: | puerarin HPLC content determination health food oil base material |
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