HPLC法测定预混剂中恩拉霉素的含量

目的采用高效液相色谱法测定饲料中恩拉霉素的含量。方法测定色谱条件为：色谱柱为C18（5um，4．6mm×250mm），流动相为0．05mol／L磷酸二氢钠-乙腈（70：30），检测波长为267nm。结果恩拉霉素A及恩拉霉素B在12．5～200ug/mL范围内线性关系良好，相关系数R。分别为0．9960和0．9945；恩拉霉素A的回收率为96．4％-101．6％，恩拉霉素B的回收率为96．8％～101．2％。恩拉霉素A及恩拉霉素B的RSD分别为0．65％和1．12％。结论该方法线性范围宽、分析时间短、样品前处理简便、定量结果准确、重复性好，为其质量控制提供了依据。

Content Determination of Enramycin in Premixes by HPLC

Objective To determine the content of Enramycin in feed by high performance liquid chromatographic method. Methods Determination of chromatographic conditions were as follow： column was C18 （5 um, 4.6 mm × 250 mm） , mobile phase was 0.05 mol/L sodium dihydrogen phosphate-acetonitrile （70：30 ,v/v）, detection wavelength was 267 nm. Results Enramycin A and Enramycin B showed a good linear relationship in the range of 12.5 - 200 ug/mL （ra2 =0.996 0, rb2 =0. 994 5）. The recovery rate of Enramycin A, and Enramycin B was 96.4% to 101.6% and 96. 8% to 101.2%. The RSD of Enramycin A and the Enramycin B was 0.65% and 1. 12%. Conclusion This method showed a wide linear range of the determination, a short analysis time, simple pre-treatment of samples, quantitative results and a good reproducibility. It could provide the basis for the quality control.