采用响应面法优化替考拉宁的层析工艺

目的对替考拉宁的层析提取工艺进行优化。方法通过对层析用树脂的筛选和单因素试验，分别考察了上样pH值、洗脱液pH、洗脱液乙醇浓度等对替考拉宁提取比的影响，确定各因素的适宜水平。在此基础上，使用Box—Behnken法通过Design—Expert软件设计响应面试验，寻找最优条件。结果替考拉宁的最佳提取工艺条件为：上样pH6．45、洗脱液pH8．00、洗脱液乙醇浓度59％。在此条件下，替考拉宁的提取比为0．9487。结论经优化，替考拉宁分离纯化的提取比提高了10．23％，产品色素符合《中国药典》的要求。

Optimization of Teicoplanin Extraction by Response Surface Analysis

Objective To optimize the extracting conditions of crude teicoplanin. Methods Different kinds of marcoporous resins were screened and single-factor designs were applied to determine the ranges of value of three related factors （sample pH, desorption agent pH, desorption agent concentration of ethanol）. Based on them, the Box-Behnken design was used to optimize the extracting conditions for teicoplanin. Results The extracting rate of teicoplanin reached to 0.948 7 when the sample pH is 6.45, pH of eluant is 8.0 and ethanol is 59%. Conclusion The extraction rate of teicoplanin was increased by 10.23% after the optimization, and the quality of teicoplanin match the requirement of ChP.