HPLC法同时测定苯甲酸水杨酸酊中苯甲酸、水杨酸的含量

目的建立测定苯甲酸水杨酸酊中苯甲酸和水杨酸含量的高效液相色谱法。方法采用Waters Symmetry ShieldRP184．6mmX150mm色谱柱；甲醇-0．05moL／L磷酸二氢钾溶液（用磷酸调pH至3．8）（45：55）为流动相；检测波长为226nm；流速0．8mL／min；柱温30℃。结果苯甲酸、水杨酸分别在0．40512～2．02560txg、0．20266～1．01178μg范围内具有良好的线性关系，回收率分别为99．8％，99．3％．RSD分别为0．1％、0．4％。结论该方法操作简便，准确，重复性好，可作为苯甲酸水杨酸酊中苯甲酸和水杨酸含量测定方法。

Determination of Benzoic Acid and Salicylic Acid in Tincture by HPLC

Objective To establish a determination method for benzoic acid and salicylic acid in tincture by high performance liquid chromatography （HPLC）. Methods K-08 Waters Symmetry Shields RP18 4.6 mm × 150 mm chromatographic column was used ; methanol-1-0.05 mol/L of potassium dihydrogen phosphate solution （ adjust to pH 3.8 with phosphoric acid） （45：55） as mobile phase; with detection wavelength of 226 nm. The flow rate was 0.8 mL/min. The column temperature was 30 ℃. Result Ben-zoic acid showed a good linearity within the range of 0.405 12-2.025 60 μg. While the mean recovery was 99.8%, RSD = 0.1%. Salicylic acid shows a good linearity between 0.202 66-1.011 78μg, while the mean recovery was 99.3%, RSD = 0.4%. Conclusion The method is simple, accurate, and reproducible, and can be used as content determination method of acid salicylic acid tincture of salicylic acid and benzoic acid.