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| 造血细胞生长因子对脐血CD34+细胞短期体外扩增的作用 | |
| 引用本文： | 叶韵斌,徐莉敏,陈志哲,林建银,陈强,周智锋,李洁羽.造血细胞生长因子对脐血CD34+细胞短期体外扩增的作用[J].福建医科大学学报,2003,37(2):147-150. |
| 作者姓名： | 叶韵斌 徐莉敏 陈志哲 林建银 陈强 周智锋 李洁羽 |
| 作者单位： | 1. 福建省肿瘤医院造血干细胞研究中心,福州,350014;福建医科大学分子医学研究中心,福州,350004 2. 福建省肿瘤医院造血干细胞研究中心,福州,350014;福建医科大学附属协和医院、福建省血液病研究所,福州,350001 3. 福建医科大学附属协和医院、福建省血液病研究所,福州,350001 4. 福建医科大学分子医学研究中心,福州,350004 5. 福建省肿瘤医院造血干细胞研究中心,福州,350014 |
| 基金项目： | 福建省科技厅科研项目，福建省卫生厅科研项目，97-Z-175，96043，， |
| 摘 要： | 目的 探讨造血细胞生长因子(HGF)的不同组合对脐血CD34 细胞短期体外扩增的作用．方法 用免疫磁珠法分高纯化脐血CD34 细胞，培养于无血清培养液中。加入不同的细胞因子，于第4，7，10，14天检测CD34 细胞百分率和有核细胞(NC)总数。结果 和对照组相比，各组的NC扩增倍数随着培养时间的延长虽不同程度的增加，干细胞在FL、TPO，GM-CSF、SCF、IL-6、G-CSF、EPO和IL-3等因子作用下，NC扩增倍数在培养第14天达高峰，为328．96倍；而CD34 细胞扩增倍数在培养第7天达最高峰，但在FL、TPO、GM-CSF、SCF，IL-6、G-CSF等因子作用下，CD34 细胞扩增倍数最大，7d后达25．23倍，而后随着培养时间的延长而逐渐下降。结论 脐血CD34 细胞在HGF作用下，扩增时间以7-10d为最佳。 |
| 关 键 词： | 基因扩增 抗原 CD34 造血细胞生长因子 胎血 |
| 文章编号： | 1672-4194(2003)02-0147-04 |
| 修稿时间： | 2003年4月29日 |
Effect of Hematopoietic Growth Factors on Short Expansion of Umbilical Cord Blood CD34+ Cells in vitro |
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| YE Yun\|bin\\{,\},XU Li\|min\\{,\},CHEN Zhi\|zhe\,LIN Jian\|yin\,CHEN Qiang\,ZHOU Zhi\|feng\,LI Jie\|yu\ .Research Center of Hematopoietic Stem Cell,Fujian Province Tumor Hospital,Fuzhou ,China .Research Center of Molecular Medicine,Fujian Medical University,Fuzhou ,China.Effect of Hematopoietic Growth Factors on Short Expansion of Umbilical Cord Blood CD34+ Cells in vitro[J].Journal of Fujian Medical University,2003,37(2):147-150. | |
| Authors: | YE Yun\|bin\\{ \} XU Li\|min\\{ \} CHEN Zhi\|zhe\ LIN Jian\|yin\ CHEN Qiang\ ZHOU Zhi\|feng\ LI Jie\|yu\ Research Center of Hematopoietic Stem Cell Fujian Province Tumor Hospital Fuzhou China Research Center of Molecular Medicine Fujian Medical University Fuzhou China |
| Institution: | YE Yun\|bin\+\{1,2\},XU Li\|min\+\{1,3\},CHEN Zhi\|zhe\+3,LIN Jian\|yin\+2,CHEN Qiang\+1,ZHOU Zhi\|feng\+1,LI Jie\|yu\+1 1.Research Center of Hematopoietic Stem Cell,Fujian Province Tumor Hospital,Fuzhou 350014,China 2.Research Center of Molecular Medicine,Fujian Medical University,Fuzhou 350004,China 3.The Affiliated Union Hospital,Fujian Medical University,Fujian Institute of Hematology,Fuzhou 350001,China |
| Abstract: | Objective To analyze the effects of hematopoietic growth factors on short expansion of umbilical cord blood CD\-\{34\}\++ cells in vitro. Methods CD\-\{34\}\++ cells were isolated from umbilical cord blood by magnetic activated cell sorting(MACS) system, cultured in a serum free culture system(SCGM). \{Being\} added different kinds of hematopoietic growth factors in the culture system, the proportion of CD\-\{34\}\++ cell and NC total number were evaluated at 4, 7, 10, 14 days. Results Compared with the control group, the NC expansion folds of the test group increased continuously during culturing and the combination of hematopoietic growth factors(FL, TPO, GM\|CSF, SCF, IL\|6, G\|CSF, EPO and IL\|3) got the best result, with 328 96 folds at 14 days. However, the CD\-\{34\}\++ cells expansion folds of every group reached the top at 7 days, and then declined gradually during culturing. The combination of hematopoietic growth factors(FL, TPO, GM\|CSF, SCF, IL\|6, G\|CSF) got the highest expansion fold, with 25 23 folds at 7 days. Conclusion There was the best expansion fold of CD\-\{34\}\++ cells in 7~10 days under the effects of some hematopoietic growth factors. |
| Keywords: | expansion antigen CD\-\{34\} hematopoietic growth factors fetal blood |
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