子宫内膜异位症病灶离体内膜组织IL-6的分泌量研究

目的:研究白细胞介素6（IL-6）在子宫内膜异位症（endometriosis,EM）发病中的作用。方法:采用酶联免疫吸附试验（ELISA）检测体外培养EM患者（12例）的异位内膜细胞和在位内膜细胞培养上清液中IL-6水平并与10例正常子宫内膜细胞作对照;以及肿瘤坏死因子α（TNF-α）对异位内膜细胞分泌IL-6的影响。结果:三种内膜细胞培养上清液中IL-6水平明显不同。异位内膜细胞分泌的IL-6显著高于相应的在位内膜（P<0.01）,而后者又明显高于正常子宫内膜（P<0.05）,即IL-6分泌量异位内膜细胞>在位内膜细胞>正常子宫内膜细胞。TNF-α作用24h后可明显促进异位内膜细胞IL-6的分泌（P<0.01）。结论:异位内膜细胞可自主分泌高水平IL-6,TNF-α进一步诱导其分泌,与EM患者腹腔免疫和炎症反应相关。EM患者在位内膜细胞分泌IL-6的活性明显升高,可能导致其异位种植。

Secretion of Interleukine-6 by Endometrial and Endometriotic Cells in Vitro

ve: To explore the role of interleukine-6 (IL-6) in the pathogenesis of endometriosis (EM). Methods: Ectopic implant endometrial (EI) and eutopic endometrial (EE) cells from 12 cases of EM along with 10 cases of normal endometrial (NE) cells were cultured, and enzyme-linked immunosorbent assay (ELISA) test was performed to determine spontaneous and tumor necrosis factor-α(TNF-α) -induced secre-tion of IL-6 in the culture medium. Results: In EI, EE and NE cells, the spontaneous production of IL-6 was significantly different, with EI>EE(P<0. 01) and EE>NE(P<0. 05). Incubation of EI cells with TNF-α(10 ng/mL) for 24 h resulted in a remarkable increase of IL-6 secretion(P<0. 01). Conclusions: Ectopic endometrial cells could secret high concentrations of IL-6 under basal and TNF-α stimulated condi-tions, which is closely connected with peritoneal inflammation of EM patients. Moreover, the characteris-tic of secreting large amounts of IL-6 of eutopic endometrium might contribute to its ectopic implants.