Human monoclonal antibodies to thyroid antigens derived by hybridization of lymphocytes from a diabetic patient |
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| Authors: | K S Tan C S Foster M DeSilva P G Byfield A R Medlen J M Wright V Marks |
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| Affiliation: | 1. Guildhay Antisera Ltd, Department of Biochemistry, University of Surrey, Guildford, United Kingdom.;2. Department of Microbiology, University of Surrey, Guildford, United Kingdom.;3. Endocrinology Research Group, Clinical Research Center, Harrow, Middlesex, United Kingdom.;4. the Department of Histopathology, St Bartholomew''s Hospital, London, United Kingdom.;1. Department of Endocrinology, Narayana Medical College, Nellore, Andhra Pradesh, 524003, India;2. Department of Endocrinology, Vydehi Institute of Medical Sciences and Research Centre, Bangalore, 560066, India;1. Rajiv Gandhi Centre for Diabetes and Endocrinology, Faculty of Medicine, J.N. Medical College, Aligarh Muslim University, Aligarh, India;2. Department of Medicine, Faculty of Medicine, Aligarh Muslim University, Aligarh, India;1. Faculty of Medicine, Tanta University, Egypt;2. Internal Medicine Department, Faculty of Medicine, Tanta University, Egypt;3. Public Health Department, Faculty of Medicine, Tanta University, Egypt;1. Department of Biochemistry, MNR Medical College, Sangareddy, MNR Nagar, Sangareddy, Telangana State, 502295, India;2. Department of General Medicine, Malla Reddy Institute of Medical Sciences, Hyderabad, India;3. Dept. of Physiology, MNR Medical College, Sangareddy, MNR Nagar, Sangareddy, Telangana State, 502295, India;4. Osmania Medical College, Hyderabad, India |
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| Abstract: | Human monoclonal antibodies to human endocrine cells have been obtained following the generation of immunoglobulin-secreting interspecies lymphocyte hybridomas. Peripheral blood lymphocytes from an adult patient presenting with acute onset, Type I, diabetes mellitus were fused in vitro with mouse myeloma cells of the NS1 cell line. Initial selection of resulting hybridomas was made by their ability to proliferate in HAT medium. Those hybridomas secreting human immunoglobulins were identified by radioimmunoassay and, thereafter, cloned at frequent intervals to ensure continued antibody production. Human monoclonal antibodies selected in this manner are being employed to identify those epitopes which are common antigenic targets during initial stages of autoimmune-mediated diabetes mellitus and associated multiple endocrinopathies. Of these antibodies, one (HML 3.22) recognizes an epitope present on the human TSH receptor and a second (HML 3.21) identifies a component of thyroglobulin. The potential value of human monoclonal antibodies as probes for analyzing autoimmune-mediated endocrine diseases is discussed. |
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