| 高效液相色谱法测定家兔血浆中赤芍_(801)的浓度 |
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| 引用本文： | 王跃生,李计萍,杜贵友,王秀荣,崔海峰,赵雍.高效液相色谱法测定家兔血浆中赤芍_(801)的浓度[J].中国中药杂志,1998,23(6):367-9, 384. |
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| 作者姓名： | 王跃生 李计萍 杜贵友 王秀荣 崔海峰 赵雍 |
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| 作者单位： | 中国中医研究院中药研究所 |
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| 摘 要： | 用反相高效液相色谱法测定家兔血浆中赤芍８０１ＰＧ浓度。采用ＮｕｃｉｅｏｓｅｌＣ１８色谱柱，甲醇∶水∶冰乙酸（４８∶５０∶２）为流动相，４羟基苯甲酸乙酯为内标物，紫外检测波长２７５ｎｍ，对ｉｖＰＧ后不同时间家兔血浆中药物含量进行测定。结果表明：ＰＧ浓度为２．５μｇ／ｍｌ的平均回收率为９６．７０％，其ＲＳＤ＝１．８３％。浓度５μｇ／ｍｌ的样品平均回收率为９８．８６％，ＲＳＤ＝１．０５％。其最低检测限为０．１μｇ／ｍｌ。
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| 关 键 词： | 赤芍_(801) 没食子酸丙酯 4-羟基苯甲酸乙酯 高效液相色谱法 |
Determination of Content of Radix Paeoniae Rubar(Chishao) 801 in Rabbit's Plasma by HPLC |
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| Wang Yuesheng,Li Jiping,Du Guiyou,Wang Xiurong,Cui Haifeng and Zhao Yong.Determination of Content of Radix Paeoniae Rubar(Chishao) 801 in Rabbit's Plasma by HPLC[J].China Journal of Chinese Materia Medica,1998,23(6):367-9, 384. |
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| Authors: | Wang Yuesheng Li Jiping Du Guiyou Wang Xiurong Cui Haifeng and Zhao Yong |
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| Institution: | Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700. |
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| Abstract: | A HPLC method was used to determine the content of Radix Paeoniae Rubar (Chishao)801 in rabbit's plasma, using Nucleosil C18 column, methanol, water and glacial acetic acid (48:50:2) as mobile phase ethye 4-hydroxybenzoate as internal standard, and UV-detector(lambda max: 275 nm, AUFS: 0.02). The average recovery was 96.70%(C: 2.5 micrograms/ml) and 98.86%(C: 5 micrograms/ml) respectively. The lowest determination limit was 0.1 microgram/ml. The method is simple, accurate, sensitive and reliable. |
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| Keywords: | Chishao 801 propyl gallate 4 hydroxybenzoate HPLC |
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