硫酸镍致雌性小鼠卵巢损伤作用

目的探讨硫酸镍致小鼠卵巢细胞凋亡的毒作用及其机制，为防治镍毒性导致的卵巢早衰提供科学依据。方法健康昆明种雌性小鼠以每次以10．0，5．0，2．5mg/kg硫酸镍（NiS04）腹腔注射染毒，0．2ml／（10g·bw），1次／d，连续12d。阴道脱落细胞学观察动情周期。摘取小鼠卵巢，制备卵巢贴片观察卵巢细胞的形态学变化，并利用流式细胞仪测定卵巢细胞细胞周期及DNA含量。结果（1）染毒组小鼠动情间期延长且不规则。（2）光学显微镜可观察到细胞凋亡的形态学变化。（3）细胞周期分析显示随染毒剂量增加，小鼠卵巢细胞呈现G0／G1期细胞阻滞且G1峰前出现Ap峰（凋亡峰。亚二倍体峰），G2／M、S期细胞比例下降；细胞凋亡率增加；细胞增殖指数（PI）降低；异倍体指数（DI）增加。结论腹腔注射硫酸镍可导致雌性小鼠卵巢损伤，其损伤作用与NiS04调控细胞周期和诱导卵巢细胞凋亡有关。

Impairment effect of nickel sulfate on ovarian cells in female mice

Objective To study the toxic effect and its mechanism of apoptosis induced by nickel sulfate in ovarian cells of female mice,and to offer scientific basis for preventing and curing premature ovarian failure(POF).Methods Healthy female Kunming mice were injected intraperitoneally with different doses of nickel sulfate(10.0,5.0,2.5 mg/kg) continuously for 12 days.Vaginal smears of mice were taken to determine estrous cycle.Morphological changes of apoptotic cells were observed by light microscopy,and the cell cycle and DNA content were analyzed by flow cytometry(FCM).Results(1)The dioestrus was prolonged and irregular in toxicant exposure groups.(2)By light microscopy,there were many morphological characteristics of apoptosis including compaction and margination of nuclear chromatin,nuclear fragments,and apoptotic bodies.(3)The result of FCM showed that nickel sulfate could result in the the arrest of G0/G1 phase and reduce the percentage of G2/M?S phase.The percentage of apoptosis and D.I was also increased while PI was reduced.Conclusion Intraperitoneal-injected nickel sulfate could induce the lesion of mice's ovary.