端粒酶检测在良恶性腹水鉴别诊断中的价值

目的探讨脱落细胞中端粒酶活性在良恶性腹水鉴别诊断中的价值。方法应用端粒酶TRAP-PCR-ELISA法分别检测60例腹水患者脱落细胞中的端粒酶活性,并与细胞学及相关肿瘤标志物进行对比分析。结果恶性腹水中的端粒酶活性水平明显高于良性腹水,在良性腹水中端粒酶活性的阳性率为10%,明显低于恶性腹水中端粒酶活性的阳性率90%,同时恶性腹水中端粒酶活性的阳性率同脱落细胞病理学检查、腹水综合指标测定(癌胚抗原、甲胎蛋白等)以及乳酸脱氢酶腹水/血清检测结果相比较,差异亦具有显著性(P<0·01)。结论脱落细胞中端粒酶活性检测可作为良恶性腹水的鉴别诊断标志。

The diagnostic value of telomerase activity in differentiating benign and malignant ascitic fluid

Objective To investigate the changes of telomerase activity in benign and malignant ascites fluid. Methods The technique of telomerase TRAP-PCR-ELISA was employed to detect telomerase activity in ascites cells from 60 patients with benign or malignant ascites fluid,the cytological diagnosis and total tumor marks(carcinoembryonic antigen, et al.)were compared. Results Telomerase activity in malignant ascites fluid was significantly higher than that in benign group. Positive rate of telomerase activity detected in malignant ascites(90%)was remarkably higher than those in benign group(10%);Compared with the cytological diagnosis and total tumor marks(carcinoembryonic antigen, et al.),telomere ferment test had higher sensitivity(90%),specificity(100%)and stability. Conclusion Telomerase activity may be an useful and sensitive mark in differential diagnosis of benign and malignant ascites fluid.