高脂高糖大鼠模型建立及血清PAI-1、APN表达的研究

目的：探讨高脂高糖大鼠模型的建立方法,并观察血清PAI-1、APN的表达。方法：利用高糖高脂饲料及给予L-NAME建立高脂高糖模型大鼠,观察模型大鼠血清葡萄糖（GLU）、总胆固醇（TC）、三酰甘油（TG）、高密度脂蛋白（HDL-C）、低密度脂蛋白（LDL-C）、纤溶酶原激活物抑制物-1（PAI-1）、脂联素（APN）的表达。结果：造模72 h后测量空腹血糖≥7.8 mmol·L-1,模型组血清GLU、TC、TG、LDL-C含量均较正常对照组明显升高（P〈0.01）,提示造模成功。高脂高糖模型大鼠血清PAI-1明显升高、APN明显下降,较正常对照组有显著性差异（P〈0.01）。结论：利用高脂高糖饮食和灌胃给予LNAME,加上链脲佐菌素（STZ）诱导,成功制作了高脂高糖症大鼠模型。高脂高糖模型大鼠血清PAI-1水平明显上调、APN水平明显下调。

Research on Establishment of Rat Model of High Fat and Sugar and the Expression of the Serum PAI-1 and APN Levels

Objective：To explore the rat model of high fat and sugar to create and serum PAI-1, APN expression. Methods：High sugar, high fat diet and given L-NAME rat model of high fat and sugar, to observe the expression of the rat model of serum GLU and TC, TG, LDL-C, HDL-C content and serum of PAI-1, APN. Results ： Modeling measured after 72h fasting plasma glucose≥ 7.8mmol / L, serum GLU and the TC,TG and LDL-C content of the model group compared with the normal control group was significantly increased （P 〈 0. 01 ）, suggesting that the model was successful. Serum of PAI-1 was significantly increased APN decreased significantly, compared with control group significant difference（ P 〈 0. 01 ）. Conclusion：Under the experimental conditions, the use of high-fat high-sugar diet and oral L-NAME, coupled with streptozotocin（STZ）-induced, and successfully produced a high-fat high-sugar disease rat model. The high fat and sugar model of serum PAI-1 levels were significantly raised ,the APN levels were significantly reduced.