乙型肝炎病毒前S1抗原与HBV-DNA和HBeAg的相关性分析

[目的]探讨乙型肝炎病毒前S1抗原与乙型肝炎病毒DNA(HBV-DNA)、乙型肝炎病毒e抗原(HBeAg)的相关性.[方法]对207例乙型肝炎患者(HBV-DNA阳性)的血清用酶联免疫法(ELISA)进行前S1抗原、HBeAg、Anti-HBe、HBcIgG的检测.用PCR荧光定量法检测HBV-DNA,同时用ELISA法对乙型肝炎病毒血清标志物阴性的80例正常健康人也进行前S1抗原的检测.[结果]207例HBV-DNA阳性的血清中,前S1抗原在HBeAg阳性而Anti-HBe阴性的血清中阳性率为70.5%,在Anti-HBe阳性而HBeAg阴性中的阳性率为68.9%,两组经χ2检验,P》0.05差异无显著性意义.在HBV-DNA低拷贝数时,前S1抗原的阳性率占25.6%,HBeAg的阳性率占11.1%;在高拷贝数时,前S1抗原的阳性率占41.1%,HBeAg的阳性率占46.3%.在低拷贝数时,前S1抗原的阳性率明显高于HBeAg.[结论]前S1抗原、HBeAg与HBV-DNA的符合率较高,对无条件检测HBV-DNA而HBeAg阴性的血清检测前S1抗原,可提示乙型肝炎病毒处于低复制的状态,如与其他乙型肝炎病毒血清标志物同时检测,对临床更有意义.

Analysis on detection results of pre S1-Ag ,HBV-DNA and HBeAg

[Objective] To discuss the duplication of hepatitis B virus(HBV) and suggest clinical diagnosis and treatment via comparison of correlation of HBV-DNA,HBeAg with HBV pre-S1 antigen.[Methods] For the 207 HBV-DNA positive patients,measure HBV pre-S1 antigen,HBeAg and Anti-HBe by ELISA and HBV-DNA by fluorescence quantitative PCR.[Results] In 207 HBV-DNA positive serum,the positive rate of HBV pre-S1 antigen in HBV-DNA positive and HBeAg positive was 70.5%;in HBV-DNA positive and Anti-HBe positive was 68.9%.The difference of the two groups was not significant.In low copies of HBV-DNA(103-106copies/ml),the positive rate of HBV pre-S1 antigen is 25.6%,HBeAg is 11.1%;in high copies of HBV-DNA,the positive rate of HBV pre-S1 is 41.1%,HBeAg is 46.3%.So in low copies,the positive of HBV pre S1 was higher than HBeAg.[Conclusions] HBV pre S1 antigen accompany with HBV-DNA.For the results of HBV-DNA positive and HBeAg negative,it is clinically significant to detect pre-S1 antigen in Hepatitis B immunological examination.