乳腺癌细胞上E-cd和CD34的表达在血管生成中的作用

目的：研究乳腺癌细胞上表皮细胞黏附分子（E-cd）的表达和癌间质的微血管密度（MVD），并分析E-cd和MVD与乳腺癌转移的关系。方法：乳腺癌标本70例,包括有癌淋巴结转移和没有癌转移的不同类型。应用常规病理组织学进行病理诊断和组织学分型，免疫组织化学方法检测E-cd和CD34的表达。结果：有淋巴结转移的乳腺癌标本其淋巴结的MVD显著高于无淋巴结转移的乳腺癌标本(P<0.05)，乳腺瘤标本的MVD同乳腺癌的组织学分型无关。当乳腺癌间质MVD级别较低时,淋巴结有无癌转移标本MVD差异无显著性(P>0.05),淋巴结有转移者淋巴结MVD高于无淋巴结转移者的淋巴结MVD(P<0.001)。结论：乳腺癌转移不仅取决于癌细胞上E-cd的基因突变,同时也取决于间质MVD；乳腺癌细胞E-cd表达情况和MVD的检测可作为诊断乳腺癌转移的一个参考指标，对于判断乳腺癌的预后有一定意义。

Effects of E-cadherin and CD34 expressions in breast cancer on angiopoiesis

Objective To study the expression of epithelial-cadherin (E-cd) in breast cancer cells and the microvessel density (MVD) in breast cancer mesenchyma, and to analyze the correlation between two factors and metastasis in breast cancer. Methods Seventy breast cancer cases with or without lymph node metastasis were selected. The routine histopathology was used to make pathological diagnosis and histological type. Immunohistochemical method was applied to detect the expressions of E-cd and CD34. Results The expression of E-cd was relative to the lymph node metastasis (P<0.05) and irrelevant to the histological types of breast cancer. The MVD in breast cancer with lymph node metastasis was significantly higher than that without lymph node metastasis, and also was irrelevant to the histological types of breast cancer. When the grade of the MVD in breast cancer mesenchyma was low, there was no difference of MVD between lymph node with metastasis and without metastasis(P>0.5),but it was relative to the MVD of lymph node(P<0.001). Conclusion Breast cancer metastasis is dependent on not only the E-cd gene mutation but also the mesenchymal MVD. Therefore, the expression of E-cd in breast cancer cells and the examination of MVD can be used as a reference marker to diagnose the breast cancer metastasis.