急性递增负荷运动对大鼠心肌JAKs及SOCS1表达的影响

目的 测定JAK家族成员以及SOCS1在运动后心肌的表达,以探讨它们在运动心肌JAK/STAT信号通路中可能的相互作用.方法 雄性SD大鼠进行递增负荷跑台运动,采用免疫组化法观察运动后0、1、3、5、12和24 h心肌JAK1、JAK3和SOCS1的表达.结果 运动后即刻大鼠心肌细胞JAK1的表达显著加强,并持续到运动后24 h;而大鼠心肌细胞JAK3及SOCS1的表达在运动后1～5h显著降低,两者在运动后12 h恢复到安静水平.结论 急性运动中,JAK1和JAK3对运动心脏功能的调节可能作用不同,SOCS1可能参与了运动心肌JAKs/STAT3信号通路的调节过程.

Expressions of JAKs and SOCS1 in myocardium induced by acute exercise

Objective To evaluate Janus kinase(JAK)s and SOCS1 expression in myocardium induced by acute exercise in order to understand the possible regulating mechanism of SOCS1 in JAK/STAT signaling pathway in exercised heart.Methods Male Sprague-Dawley rats were submitted to continuous graded treadmill running,then were sacrificed at 0,1,3,5,12 and 24 h respectively after the exercise.JAK1,JAK3 and SOCS1 expressions in myocardium were determined with immunohistochemistry.Results JAK1 expression significantly increased immediately after running in comparison with control group,peaked at 1~5 h,and remained high at 24 hr after exercise;in contrast,JAK3 and SOCS1 expressions significant decreased from 1 h to 5 h after running,then gradually back to sedentary level 12 h after exercise.Conclusion Acute exercise induced different responses of JAK1 and JAK3,which indicated the different functions of JAKs in exercised heart,SOCS1 may involve in regulating JAK/STAT3 signal pathway in the exercised heart.