实时荧光定量PCR检测人巨细胞病毒感染的临床意义分析

目的分析实时荧光定量聚合酶链反应(PCR)检测在人巨细胞病毒(HCMV)感染中的应用价值。方法选取363例疑似HCMV感染患儿及50例健康儿童,均采集血样进行实时荧光定量PCR检测。分析荧光定量PCR检测结果 ,并比较不同性别、不同年龄HCMV感染的阳性检出情况。结果实时荧光定量PCR检测显示, HCMV感染患儿中32例为阳性,阳性率为8.82%;健康儿童中1例为阳性,阳性率为2%。阳性患儿的HCMV载量在5.0×102~1.0×104 copies/ml。HCMV感染患儿阳性率高于健康儿童,差异具有统计学意义(P<0.05)。188例男性CMV感染患儿的阳性率为9.04%(17/188), 175例女性CMV感染患儿的阳性率为8.57%(15/175),不同性别HCMV感染患儿阳性率比较,差异无统计学意义(P>0.05)。152例年龄<1岁CMV感染患儿的阳性率最高,为14.5%;其次为1~3岁患儿,阳性率为6.9%;109例>3岁患儿的阳性率最低,为2.8%;不同年龄段CMV感染患儿的阳性率比较差异具有统计学意义(P<0.05)。结论 <1岁儿童由于机体的免疫力和抵抗力较弱,容易出现HCMV感染的情况,因此需要借助实时荧光定量PCR技术进行筛查,从而尽早诊断和治疗,能够避免严重并发症的出现。

Analysis of clinical significance of real-time fluorescence quantitative PCR in detection of human cytomegalovirus infection

Objective To analyze the value of real-time fluorescence quantitative polymerase chain reaction(PCR) in detection of human cytomegalovirus(HCMV) infection. Methods A total of 363 suspected HCMV infection children and 50 healthy children, and their blood samples were collected for real-time fluorescent quantitative PCR detection. The results of fluorescent quantitative PCR detection were analyzed, and the positive rates of different genders and different ages were compared. Results According to real-time fluorescent quantitative PCR, there were 32 positive cases, with a positive rate of 8.82%;1 case of healthy children was positive, with a positive rate of 2%. The HCMV load of positive children was 5.0 ×102-1.0×104 copies/ml. The positive rate of HCMV children was higher than that of healthy children, and the difference was statistically significant(P<0.05). The positive rate of 188 male children with CMV infection was 9.04%(17/188), and the positive rate of 175 female children with CMV infection was 8.57%. There was no significant difference in the positive rate of HCMV infection between male and female(P>0.05). The highest positive rate was 14.5% in 152 children with CMV infection< 1 year of age, followed by 6.9% in children 1-3 years of age, and the lowest was 2.8% in 109 children > 3 years of age, and the difference was statistically significant(P<0.05). Conclusion <1 year old children are vulnerable to HCMV infection due to weak body immunity and resistance, so they need to be screened with the help of real-time quantitative PCR technology. Thus avoid serious complications by early diagnosis and treatment.