苯扎氯铵对大鼠结肠神经及平滑肌的影响

目的研究苯扎氯铵对大鼠结肠神经及平滑肌的影响。方法8~9周龄SD大鼠50只,随机分为两组。氯胺酮麻醉下开腹,实验组用0.1%苯扎氯铵处理大鼠降结肠浆膜40min,温盐水冲洗后关腹,对照组用生理盐水代替苯扎氯铵。分别于术后1、2、3、4、8周取处理段结肠行组织学检查,反转录-聚合酶链反应(RT-PCR)检测乙酰胆碱酯酶(AchE)、巢蛋白(nestin)、乙酰胆碱M3受体(Chrm3)的表达,循环水浴药理学检测平滑肌收缩功能变化。结果苯扎氯铵处理后1周,大鼠结肠神经节细胞明显减少、空泡变,3周后完全消失;结肠平滑肌对乙酰胆碱敏感性增强。结论采用0.1%苯扎氯铵可使大鼠结肠产生去神经作用,结肠平滑肌对乙酰胆碱敏感性增强。

Effect of benzalkonium chloride on colonic nerve and smooth muscle of rat

ObjectiveTo investigate the effect of benzalkonium chloride (BAC) on colonic nerve and smooth muscle of rat.MethodsFifty Sprague Dawley rats,89 weeks old,were randomly divided into two groups:treatment group (n=25) and control group (n=25).All animals were operated upon under ketamine anesthesia.In treatment group,0.1% benzalkonium chloride (BAC) was applied onto the serosa for 40 minutes, and then the colon was washed well with 0.9% normal saline.For the control group,0.9% saline was applied using the same method as in treatment group.Microscopic observations,the expression of AchE,nestin,Chrm_3 by RT-PCR and pharmacologic examinations for contraction of colonic smooth muscle were performed at an interval of 1w,2w,3w,4w and 8w after operation.ResultsHistologic examinations revealed obvious reduction and vacuolation of ganglion cells 1 week after BAC treatment,with a complete disappearence in 3 weeks after BAC treatment.Pharmacologic examinations indicated the colonic smooth muscle got an enhanced sensitivity to acetylcholine 1 week after BAC treatment.ConclusionThe denervation of colon in rat can be produced by applying 0.1% benzalkonium chloride onto the serosa of colon,and the colonic smooth muscle becomes supersensitive to acetylcholine.