HBO对大鼠局灶性脑缺血损伤及海马中NO含量的影响

目的研究高压氧（HBO）对大鼠局灶性脑缺血后梗死体积及海马中一氧化氮（NO）含量的影响。方法：用线栓塞SD大鼠右大脑中动脉，造成局灶性脑缺血再灌注模型，在HBO和高压空气（HBA）治疗下，观察不同时间点脑梗死体积和海马中NO含量的改变。结果：脑缺血1h后出现明显梗死灶，且随再灌注时间延长而逐渐扩大，海马中NO含量明显增高（P＜0．01）；经HBO治疗后，明显地抑制梗死灶的扩大（P＜0．05），NO含量明显降低（P＜0．05）；HBA对脑梗死灶扩大和NO含量无明显抑制作用（P＞0．05）。结论：HBO可抑制大鼠局灶性脑缺血梗死灶的扩大和降低NO含量。

Effects of Hyperbaric Oxygen on Focal Cerebral Ischemic Injury and Nitric Oxide Content in Hippocampus of Rats

Objective To investigate the effects of hyperbaric oxygen(HBO) on focal cerebralischemic injury and nitric oxide (NO) content in hippocampus of rats. Methods: The right middle cere-bral artery(MCA) was occluded in a SD rat by the insertion of a nylon thread, and then the model of fo-cal ischemic brain injury for reperfusion was induced. The infarct volume and the NO content were mea-sured after the treatment of HBO and hyperbaric air(HBA) at 30 min, 1 , 2 and 4 h. Results: One hourafter the cerebral ischemia, an obvious infarct developed. It enlarged gradually with the time of reperfu-sion and the NO content increased significantly in rats' hippocampus (P < 0.01 ). After being treatedwith the HBO, the infarct volume was controlled significantly (P < 0.05) and the NO content decreasedsignificantly (P < 0.05). However, the HBA did not have the significant effects on controlling the in-farction and NO content (P > 0.05). Conclusion: HBO can control the foci of the focal cerebral infarc-tion and decrease the NO content in rats' hippocampus.