p27、CyclinD1和Ki-67在乳腺导管癌中的表达及相关性分析

目的:探讨乳腺导管癌中p27,CyclinD1和Ki-67蛋白的表达及意义。方法:采用免疫组化法探讨20例导管内癌、20例微浸润导管癌和40例浸润性导管癌组织中p27,CyclinD1和Ki-67蛋白的表达。结果:①在导管内癌、微浸润导管癌和浸润性导管癌组织中p27蛋白的高表达率呈递减趋势,CyclinD1和Ki-67的高表达率呈递增趋势。②p27,CyclinD1和Ki-67的高表达率在导管内癌、微浸润导管癌和浸润性导管癌中的差异有统计学意义(P<0.05);③不同阶段乳腺导管癌中p27与CyclinD1/Ki-67的高表达率均呈负相关。结论:p27在乳腺导管癌的发生发展中可能起明显的抑制作用,联合检测p27与CyclinD1/Ki-67对反映乳腺导管癌的恶性程度以及预后可能更为客观。

Expression and correlation analysis of p27, CyclinD1 and Ki-67 in breast ductal carcinomas

Objective:To explore the expression and significances of p27,CyclinD1 and Ki-67 in breast ductal carcinomas.Methods:The expression of p27,CyclinD1 and Ki-67 was detected in 20 cases of intraductal carcinomas,20 cases of micro-invasive ductal carcinomas and 40 cases of invasive breast ductal carcinomas by immunohistochemistry.Results:①The high-expression rates of p27 in intraductal carcinomas,micro-invasive ductal carcinomas and invasive breast ductal carcinomas were gradually decreased.The high-expression rates of CyclinD1 and Ki-67 were gradually increased.②The high-expression rates of p27,CyclinD1 and Ki-67 in intraductal carcinomas,micro-invasive ductal carcinomas and invasive breast ductal carcinomas had significance of statistics(P<0.05).③The high-expression rates of p27 and CyclinD1/Ki-67 protein were inversely correlated in different stages of breast ductal carcinomas.Conclusion:Abnormal high-expression of p27 may play an suppressive role in initiation and development of breast ductal carcinomas.The combined analysis of p27 and CyclinD1/Ki-67 expression is considered to be more reliable for assessing the histopathologic grades and prognosis of breast ductal carcinomas.