| IL-18对人早孕滋养细胞MMP-9和TIMP-1 mRNA表达的影响 |
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| 引用本文： | 解其贵,王海霞,杨瑜.IL-18对人早孕滋养细胞MMP-9和TIMP-1 mRNA表达的影响[J].同济大学学报(医学版),2013,34(1):45-47. |
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| 作者姓名： | 解其贵 王海霞 杨瑜 |
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| 作者单位： | 复旦大学附属金山医院妇产科;上海市公共卫生临床中心科研科 |
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| 摘 要： | 目的研究不同浓度白细胞介素18(interleukin-18,IL-18)对人早孕滋养细胞MMP-9和TIMP-1表达的影响。方法分离、培养早孕期滋养细胞,以不同浓度的IL-18作用于体外培养的细胞滋养细胞。PCR(Real-TimePCR)检测MMP-9及TIMP-1 mRNA的表达。结果随着IL-18浓度的增高,滋养细胞中MMP-9 mRNA的表达水平下降(P<0.01),TIMP-1 mRNA的表达水平则相反(P<0.05)。结论 IL-18能减弱滋养细胞表达MMP-9的能力,而增强TIMP-1的表达,IL-18可能通过节制性调节MMPs/TIMPs平衡,进而影响滋养细胞的侵袭。
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| 关 键 词： | 白细胞介素18 滋养细胞 基质金属蛋白酶9 基质金属蛋白酶组织抑制物-1 |
Effect of IL-18 on expression of MMP-9 and TIMP-1 in trophoblasts of human first trimester gestation |
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| XIE Qi-gui,WANG Hai-xia and YANG Yu.Effect of IL-18 on expression of MMP-9 and TIMP-1 in trophoblasts of human first trimester gestation[J].Journal of Tongji University(Medical Science),2013,34(1):45-47. |
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| Authors: | XIE Qi-gui WANG Hai-xia and YANG Yu |
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| Institution: | 1.Dept.of Gynaecology and Obstetrics,Jinshan Hospital,Fudan University,Shanghai 200540,China; 2.Dept.of Scientific Research,Shanghai Public Health Clinical Center,Shanghai 201508,China) |
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| Abstract: | Objective To investigate the effect of IL-18 on the expression of MMP-9 and TIMP-1 in trophoblasts of human first trimester gestation. Methods The trophoblasts of human first trimester gestation were cultured and treated with different concentrations of IL-18. The expression of MMP-9 and TIMP-1 was detected by Real-Time PCR. Results The expression of MMP-9mRNA was declined significantly with the increased concentrations of IL-18, while the expression of TIMP-lmRNA was increased. Conclusion IL-18 can down-regulate the expression of MMP-9mRNA and up-regulate the expression of TIMP-1 mRNA, which may affect the invasion of trophoblasts. |
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| Keywords: | interleukin-18 trophoblast matrix metalloproteinsaes-9 tissue inhibitor ofmetalloproteinase-1 |
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