头孢西丁诱导产ACT-1型AmpC酶大肠埃希菌的比较蛋白质组学研究

目的研究产AmpC酶大肠埃希菌在头孢西丁诱导前后的蛋白质谱差异,筛选有价值的靶位。方法提取产ACT-1AmpC酶大肠埃希菌在头孢西丁诱导前后的全菌蛋白,应用双向凝胶电泳技术分析和Blue silver法染色。凝胶图像分析后,对差异蛋白质进行MALDI-TOF质谱分析。结果在所鉴定的8个差异蛋白当中,AmpC酶、外膜蛋白II和转座酶表达显著上调,而磷酸转移酶系统的葡萄糖特异性组分IIA蛋白、抗碲酸盐蛋白、丙三醇磷酰基二酯酶、硫醇过氧化物酶及细菌分化蛋白FtsZ表达下调。结论所鉴定的这些差异蛋白将为阐明产AmpC酶大肠埃希菌耐药产生的机制提供线索,也为筛选具有潜在价值的靶位提供理论依据。

Comparative proteomics analysis of Escherichia coli producing ACT-1 type class C beta-lactamase induced with Cefoxitin

Objective To study protein alteration profile between before and after the AmpC production of E.coli induced by cefoxitin.Methods The extracted proteins were separated by 2-Di mensional electrophoresis(DE) and stained with blue silver staining.After gel image analysis,the differential staining spot were identified by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) technology.Results Of the identified eight differential proteins, AmpC beta-lactamase, outer membrane protein II,transposase were up-regulated after induction.While glucose-specific component IIA of the PTS system, tellurite resistance,glycerophosphoryl diester phosphordiesterase, thiol peroxidase, cell division protein FtsZ were down-regulated after induction.Conclusion These differentially expressed proteins give some clues to elucidate the the resistant mechanism of E.coli producing AmpC,and provide the basis of searching for potential target.