外源性溶血卵磷脂对急性胰腺炎大鼠血脑屏障通透性的影响

目的　探讨溶血卵磷脂(LPC)对胰腺炎(AP)大鼠血脑屏障(BBB)通透性的影响。方法　SD大鼠制成急性胰腺炎模型后随机分为: ( 1 )实验组,从大鼠的尾静脉注入LPC; ( 2 )对照I组,从尾静脉注入生理盐水; ( 3 )对照II组(假手术组),不制备AP模型,仅从尾静脉注入LPC。7 ~ 1 0d后应用辣根过氧化物酶-二氨基联苯氨显色法(HRP DAB法)检测3组大鼠BBB的通透性。结果　实验组HRP有局部血管外渗出,显示BBB通透性明显升高,对照I,II组BBB升高不明显,两者具有统计学差异(P< 0. 0 1, P< 0. 0 5 )。结论　LPC能增加胰腺炎大鼠BBB的通透性,可能与AP的胰性脑病发生有关。

Effect of ectogenic lysolethcin choline on blood brain barrier permeability in rats with acute pancreatitis

Objective To explore the effect of lysolecithin choline (LPC) on blood-brain barrier(BBB) permeability in rats with acute pancreatitis. Methods Acute pancreatitis rat model was produced and rats were randomly divided into:(1)Test group-AP, rats received LPC by tail vein injection; (2)control group I, AP rats were given normal saline by tail vein injection; (3)control group II, sham operation without AP, but LPC was given by tail vein injection.Horseradish peroxidase(HRP) was used as a tracer to determine BBB permeability 7~10 days later.Results The test group showed local extravascular effusion of HRP, indicating that BBB permeability was markedly increased,while both control groups showed no apparent increase of BBB permeability,which were statistically significant(P<0.01,P<0.05). Conclusions LPC can increase BBB permeability of rats with acute pancreatitis,and this may be related to the development of pancreatic encephalopathy.