脉冲磁场对多药耐药细胞K562/ADR的逆转作用

目的:研究脉冲磁场(PMF)对多药耐药(MDR)肿瘤细胞K562/ADR的逆转作用及机制。方法:MTT法测定敏感株K562/S和耐药株K562/ADR对多柔比星(ADR)的IC50,观察PMF对肿瘤细胞增殖的影响。测定不同频率PMF照射的K562/ADR耐药细胞株对ADR的IC50,分析PMF的逆转效果。用流式细胞术(FCM)检测细胞内ADR的浓度。结果:K562/ADR细胞的耐药倍数为29.36,PMF对耐药株肿瘤细胞增殖无影响。相同磁场强度、不同频率的PMF对耐药株K562/ADR的IC50影响不同,在相同的时间里,70 Hz的PMF影响作用最明显。70 HzPMF处理的K562/ADR细胞中药物浓度高于对照组。结论:PMF可通过提高细胞内的药物浓度,对肿瘤细胞K562/ADR的MDR具有逆转作用;逆转作用的大小与脉冲磁场的频率有关,低频率的PMF比高频率的PMF的逆转效果更明显。

Reversal effect of pulsed magnetic field on the multidrug-resistant cell line K562/ADR

Objective: To investigate the reversal effects of pulsed magnetic fields(PMF) on the drug resistant cell line K562/ADR in vitro and their mechanism.Methods: MTT assay was used to determine the IC50 of ADR on K562/S and K562/ ADR cell lines,the detriment of PMF on the K562/ADR cell line and the reversal effects of the drug resistance after treatment with different frequencies of PMF.Flow cytometry(FCM) was applied to the assessment of concentration of intracellular ADR.Results: The drug-resistance multiple of the K562/ADR cell line was 29.36.PMF did not affect the growth of the K562/ADR cell line.Different frequencies of PMF produced different effects on the IC50 of the K562/ADR cell line.In the same condition,70Hz PMF produced the most significant effect and increasea the concentration of intracellular ADR.Conclusion: PMF can reverse the multidrug resistance of K562/ADR by enhancing the drug concentration in K562/ADR cells,and its frequency is correlated with its reversal effect:low fregucncy produces better effect.