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| 银杏叶对低密度脂蛋白氧化修饰抑制作用的时效研究 | |
| 引用本文： | 黄沛力,冯高谦,张淑华,王晖,金有豫. 银杏叶对低密度脂蛋白氧化修饰抑制作用的时效研究[J]. 卫生研究, 2004, 33(4): 453-454,M003 |
| 作者姓名： | 黄沛力 冯高谦 张淑华 王晖 金有豫 |
| 作者单位： | 1. 首都医科大学公共卫生与家庭医学学院,北京,100054 2. 首都医科大学研究生院 3. 首都医科大学基础医学院 |
| 摘 要： | 目的 探讨银杏叶不同有效成分 [总提取物EGB76 1 ;银杏叶总黄酮 (GF) ;银杏叶总内酯 (GT) ]对Fe2 + 或Cu2 + 诱导的人血浆低密度脂蛋白 (LDL)氧化修饰的抑制作用与时间的变化关系。方法 使用荧光分光光度法测定了LDL在Fe2 + 或Cu2 + 诱导下 ,分别加入上述三种有效成分 ,作用 1、2、4、6h后自身荧光物质(LF)、丙二醛 (MDA)和维生素E(VitE)含量。结果 三种有效成分对LF和MDA的生成及VitE的消耗有抑制作用 ,抑制作用在 4h内随作用时间延长逐渐明显 ,6h时抑制作用明显减弱。结论 银杏叶对低密度脂蛋白氧化修饰抑制作用具有一定的时效关系 |
| 关 键 词： | 低密度脂蛋白 银杏叶 黄酮 内酯 铜 铁 |
| 文章编号： | 1000-8020(2004)04-0453-03 |
Effect of Ginkgo biloba L leaves on oxidation of human low density lipoproteins in vitro |
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| Huang Peili,Feng Gaoqian,Zhang Shuhua,Wang Hui,et al.. Effect of Ginkgo biloba L leaves on oxidation of human low density lipoproteins in vitro[J]. Journal of hygiene research, 2004, 33(4): 453-454,M003 | |
| Authors: | Huang Peili Feng Gaoqian Zhang Shuhua Wang Hui et al. |
| Affiliation: | Faculty of Public Health, Capital University of Medical Sciences, Beijing 100054, China. |
| Abstract: | OBJECTIVE: To study the effects of Ginkgo biloba L leave on oxidation of human low density lipoproteins (LDL) induced by Fe2+ or Cu2+ in vitro. METHODS: LDL was oxidized by Fe2+ or Cu2+ for 1, 2, 4, 6h, while it was protected by extracts from leaves of Ginkgo biloba L. ( EGB761), ginkgo flavonids(GF) and ginkgo terpenlactones (GT). Lipofusion (LF), malondialdehyde (MDA) and vitamin E (VitE) were measured as the index of the oxidation of LDL by means of Fluorescence spectroscopy methods. RESULTS: Each extract can inhibit the oxidation of LDL, the inhibition of each extract is increasing during the first 4 hours and subsequently decrease, after 6 hours all the inhibitor have almost lost their ability on antioxidation. CONCLUSION: EGB761, GF and GT are effective antioxidant and the anti-oxidation vary with time. |
| Keywords: | low density lipoprotein Ginkgo biloba L flavonids terpenlactones copper iron |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |