结直肠癌患者不同组织中K-ras基因研究

目的 研究结直肠癌患者不同组织中K-ras基因的临床意义。方法 采用实时荧光定量PCR方法检测患者原发灶组织100例、转移淋巴结组织64例、远处转移灶13例、腺瘤组织19例中K-ras基因突变情况。结果 结直肠癌原发灶、转移淋巴结组织、远处转移灶、腺瘤组织的K-ras基因检出率分别为100%、92.2%、92.3%、89.5%;K-ras基因突变率分别为39.0%、30.5%、33.3%、17.6%。主要突变基因第12编码子34位 G>T, G>A, G>C, 35位 G>A, G>T, G>C;第13编码子 37位 G>C, 38位 G>A等8种基因发生突变。结论 结直肠癌原发灶、转移淋巴结组织、远处转移灶的K-ras基因突变率基本一致, 而腺瘤组织K-ras基因突变率明显低于原发灶、转移淋巴结组织、远处转移灶组织。K-ras基因突变在结直肠癌的发生、发展中起重要作用, 这对结直肠癌的防治有重要的指导意义。

Research of K-ras Gene of Colorectal Cancer at Different Tissue

Objective The Research of K-ras gene of colorectal cancer at different tissue. Methods One-hundred primary foci, 64 metastatic lymph nodes, 13 metastatic remoter organs and 19 colorectal adenoma from colorectal cancer patients were collected Real-time fluorescence quantitative PCR were performed to detct K-ras mutations results were analyzed. Results The frequency of K-ras detct in primary foci, metastatic lymph nodes, metastatic remoter organs and colorectal adenoma were 100%, 92.2%, 92.3% and 89.5%. The frequency of K-ras mutation in primary foci, metastatic lymph nodes, metastatic remoter organs and colorectal adenoma were 39.0%, 30.5%, 33.3% and 17.6%. The most common mutation of codon was 12 (G>T, G>A, G>C, G>A, G>T, G>C) and codon 13 (G>C, G>A). Conclusion The detection of K-ras mutations in primary foci, metastatic lymph nodes, metastatic remoter organs was concordant. K-ras mutations play an important role in the carcinogenesis and development of colorectal cancer.