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| 低温胁迫对新疆紫草悬浮细胞次生代谢物的影响 | |
| 引用本文： | 谢腾,王升,张山山,刘谈,郭兰萍.低温胁迫对新疆紫草悬浮细胞次生代谢物的影响[J].中草药,2015,46(10):1525-1532. |
| 作者姓名： | 谢腾 王升 张山山 刘谈 郭兰萍 |
| 作者单位： | 1. 西南交通大学生命科学与工程学院,四川成都610031;中国中医科学院中药资源中心,北京100700;道地药材国家重点实验室,北京 100700 2. 中国中医科学院中药资源中心,北京100700;道地药材国家重点实验室,北京 100700 3. 中国中医科学院中药资源中心,北京100700;道地药材国家重点实验室,北京 100700;武汉轻工大学,湖北武汉430023 |
| 基金项目： | 国家自然科学基金资助项目(81130070, 81072989);国家科技支撑项目(2012BAI29B02, 2012BAI28B002) |
| 摘 要： | 目的通过考察低温胁迫后新疆紫草悬浮细胞次生代谢物量的变化,探讨道地产区特有的环境因子对新疆紫草的作用。方法 4℃低温胁迫新疆紫草悬浮细胞24h后,HPLC法分别测定胁迫前(计为0h)和胁迫后12、24、48、72、168h5个时间点迷迭香酸、紫草酸B、紫草呋喃A、紫草呋喃E、乙酰紫草素、脱氧紫草素、异丁酰紫草素、β,β'-二甲基丙烯酰紫草素、异戊酰紫草素和总紫草素类化合物的量。结果低温胁迫后不同时间点同类化合物的变化趋势基本一致,但其量差异显著,除β,β'-二甲基丙烯酰紫草素外,其他化学成分量的最大值均出现在低温胁迫组。结论低温胁迫可以促进新疆紫草悬浮细胞积累次生代谢物,并对揭示新疆紫草道地性形成的机制有重要作用。 |
| 关 键 词： | 低温胁迫 新疆紫草 次生代谢物 迷迭香酸 紫草酸B 紫草呋喃A 紫草呋喃E 乙酰紫草素 脱氧紫草素 异丁酰紫草素 β β'-二甲基丙烯酰紫草素 异戊酰紫草素 |
| 收稿时间： | 2014/12/13 0:00:00 |
Effects of low temperature stress on secondary metabolites of Arnebia euchroma suspension cells |
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| XIE Teng,WANG Sheng,ZHANG Shan-shan,LIU Tan and GUO Lan-ping.Effects of low temperature stress on secondary metabolites of Arnebia euchroma suspension cells[J].Chinese Traditional and Herbal Drugs,2015,46(10):1525-1532. | |
| Authors: | XIE Teng WANG Sheng ZHANG Shan-shan LIU Tan and GUO Lan-ping |
| Institution: | School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China;National Resource Center of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;State Key Laboratory of Daodi Herbs, National Resource Center of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Resource Center of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;State Key Laboratory of Daodi Herbs, National Resource Center of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Resource Center of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;State Key Laboratory of Daodi Herbs, National Resource Center of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Wuhan Polytechnic University, Wuhan 430023, China;National Resource Center of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;State Key Laboratory of Daodi Herbs, National Resource Center of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Resource Center of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;State Key Laboratory of Daodi Herbs, National Resource Center of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
| Abstract: | |
| Keywords: | low temperature stress Arnebia euchroma (Royle) Johnst secondary metabolites rosmarinic acid lithospermum acid shikonofuran A shikonofuran E acetylshikonin deoxyshikonin β β'-dimethylacryl shikonin isovalerylshikonin |
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