生长反应因子与大鼠颈动脉损伤后内膜增生

背景：经皮冠状动脉介入治疗后再狭窄仍是影响介入治疗远期疗效的严重的临床问题。目的：在大鼠颈动脉球囊损伤模型中，探讨早期生长反应因子诱骗寡脱氧核苷酸对损伤后的血管内膜的影响，并初步探讨其分子机制。方法：利用2FFogarty导管损伤Wistar大鼠颈动脉，构建大鼠球囊损伤模型，在转染试剂Fugene6介导下经血管腔内转染生长反应因子诱骗寡脱氧核苷酸，苏木精一伊红染色法观察血管内膜增生情况，免疫组化法检测CyclinD1，观察其表达情况。结果与结论：早期生长反应因子诱骗寡脱氧核苷酸可以显著抑制大鼠颈动脉球囊损伤后血管内膜增生，同时也可以下调大鼠颈动脉球囊损伤后表达显著增加的CyclinD1。说明生长反应因子诱骗寡脱氧核苷酸可能通过抑制CyclinD1的表达，使细胞周期停滞，从而抑制损伤后的大鼠颈动脉内膜的增生。

Decoy oligodeoxynucleotides and neointimal hyperplasia following balloon injury of rat common carotid artery

BACKGROUND： Restenosis following percutaneous coronary intervention is still a clinically serious problem which negatively affects the long-term therapeutic benefit of percutaneous coronary intervention OBJECTIVE： To investigate the effect of Egr-1 decoy oligodeoxynucleotides via intracerebroventricular injection on the neointima after carotid balloon injury and to primarily study its mechanism of inhibiting neiointimal hyperplasia, thus providing a new prospect to find a new target of vascular remodeling and restenosis. METHODS： Endothelial denuded carotid models were prepared in rats by balloon withdrawal injury with the help of 2F Fogarty. Then Egr-1 decoy oligodeoxynucleotides were injected into injured vascular subsection which was mediated by Fugene6 transfection reagent. The extent of neointimal hyperplasia and the expression of Cyclin D1 were detected by hematoxylin-eosin staining and immunohistochemistry, respectively.RESULTS AND CONCLUSION： NeointimaL hyperplasia was significantly inhibited by Egr-1 decoy oligodeoxynucleotides. The expression of Cyclin D1, which was dramatically increased after balloon injury of rat common carotid artery, was significantly down-regulated by Egr-1 decoy oligodeoxynucleotides. Egr-1 decoy oligodeoxynucleotides may inhibit neointimal hyperplasia following balloon injury of rat common carotid artery via down-regulation of Cyclin DI.