组织工程角膜内皮层移植的研究与应用

背景：组织工程角膜内皮可以克服角膜内皮移植的短缺并能够改善角膜内皮细胞的质量,其应用前景十分广阔。因此,寻找一种理想的重建角膜内皮方法是组织工程角膜研究领域的重要课题和研究热点。目的：总结并讨论组织工程角膜内皮重建的研究进展。方法：应用计算机检索PubMed数据库（http：//www.ncbi.nlm.nih.gov/PubMed）相关文章,检索时间为2000至2012年,检索词为＂corneal endothelial cell,transplantation,tissue engineering＂,并限定文章语言种类为英语,然后再从中筛选出与主题相关的部分文献。结果与结论：初检得到163篇文章,按主题相关性对文献进一步筛选,最终纳入44篇文章。目前构建单层角膜内皮层的研究较多,也取得了巨大的成绩,但是仍没有一种材料或重建方式可以完全符合临床应用的组织工程的人角膜内皮。每一种材料和方法都各有优缺点,克服这些缺点以及观察在体内的长期影响是今后的发展方向。

Transplantation of tissue-engineered corneal endothelium

BACKGROUND： Tissue-engineered corneal endothelium can overcome the shortages of donor corneas for transplantation and also improve the quality of corneal endothelial cells. Finding a kind of ideal reconstructed corneal endothelium is very important in the field of tissue-engineered cornea.OBJECTIVE： To review and discuss the current research in the reconstruction of tissue-engineered corneal endothelium.METHODS： The PubMed database （http：//www.ncbi.nlm.nih.gov/PubMed） was retrieved by computer to search the relevant literature published between 2000 and 2012 using the key words of “corneal endothelial cell, transplantation, tissue engineering” in English. Then, the paper was further analyzed and reviewed in line with the theme.RESULTS AND CONCLUSION： A total of 163 papers were searched. At last, 44 papers were selected according to the titles and objectives. So far, many kinds of biomaterials and methods have been used for reconstruction of tissue-engineered corneal endothelium. But many difficulties and problems must be solved in the clinical practice of tissue-engineered corneal endothelium. Every material and method for tissue-engineered corneal endothelium has its own advantages and disadvantages, so further research is to improve this disadvantage and investigate the long-term effects in vivo.