脾脏提取DNA与人类白细胞抗原配型实验方法

背景：人类白细胞抗原配型结果报告准确与否,直接影响供受者之间的配对选择,而配型样本的好坏则影响结果的优劣。目的：建立脾脏提取DNA进行人类白细胞抗原配型实验的实验技术平台。方法：通过从脾脏分离淋巴细胞提取DNA进行人类白细胞抗原配型实验,并同正常的和严重溶血的外周血提取DNA进行人类白细胞抗原配型的实验进行比较。结果与结论：通过脾脏和正常外周血提取的DNA经扩增、电泳后均获得了完整的DNA电泳图,脾脏比正常外周血的电泳图更清晰,而严重溶血的标本未能获得清晰完整的DNA电泳图。实验成功建立了脾脏提取DNA进行人类白细胞抗原配型实验的实验技术平台。

DNA extraction from the spleen in human leukocyte antigen matching

BACKGROUND： Whether human leukocyte antigen matching results are accurate or not may directly affect the pairing choice between the donors and recipients. Moreover, the quality of the matching samples may influence the pros and cons of the results. OBJECTIVE： To establish an experimental technology platform for DNA extraction from the spleen in human leukocyte antigen matching. METHODS： Lymphocytes were isolated and DNA was extracted from the spleen for the human leukocyte antigen matching, compared with DNA extracted from the whole blood which was normal peripheral blood or hematolytic sample. RESULTS AND CONCLUSION： Intact electrophoretogram of DNA extracted from the spleen and normal peripheral blood was obtained after amplification and electrophoresis, and the electrophoretogram of DNA extracted from spleen was clearer than that extracted from the normal peripheral blood. However, the intact electrophoretogram of DNA extracted from hematolytic sample was not obtained. An experimental technology platform for DNA extraction from the spleen in human leukocyte antigen matching has been successfully established.