神经营养因子3纳米微球载体基因工程转染许旺细胞

背景：纳米微球基因转染技术携带或增强种植细胞可持续稳定延长其释放，保持其活性和作用时间。目的：观察纳米微球载体转染神经营养因子3基因修饰的许旺细胞对坐骨神经缺损的促进和修复作用。方法：采用纳米微球载体将神经营养因子3基因转染入体外培养的新生Wistar大鼠许旺细胞。取Wistar成年大鼠80只制作坐骨神经缺损模型，将4种不同的移植物注入细胞外基质凝胶一聚乳酸聚羟基乙酸共聚物管桥接管内：空白对照组未注入其他任何物质，细胞组注入许旺细胞，基因组注入神经营养因子3基因，实验组注入神经营养因子3基因修饰的许旺细胞。结果与结论：术后坐骨神经及肌纤维横截面积染色比较，实验组优于细胞组、基因组（P〈0．01），细胞组、基因组均优于空白对照组（P〈0．01），细胞组、基因组组间比较差异无显著性意义（P〉0．05）。表明神经营养因子3基因转染许旺细胞移植，可相互促进，再造神经再生的微环境，促进并修复缺损坐骨神经缺损。

Transfection of Schwann cells with neurotrophic factor-3 nanoparticles

BACKGROUND： The technology of nanoparticle carrier transferring gene can carry or enhance plant cel sustainable stability, extend its release and maintain its activity and duration. OBJECTIVE： To explore the effects of nanoparticle carrier with neurotrophic factor-3 on transfection of Schwann cells in facilitating and repairing sciatic nerve defects.METHODS： The neurotrophic factor-3 genes were transfected to the Schwann cells of newborn Wistar rats with nanoparticle carrier. Eighty adult Wistar rats were selected to make the sciatic nerve defect model. Then four kinds of grafts were injected into the pipe bridge takeover of extracellular matrix gel-poly（ lactide-co-glycolide）： the blank control group was not injected with other substances, the cell group was injected with Schwann cells, the gene group was injected with neurotrophic factor-3 gene, and the experimental group was injected with neurotrophic factor-3 gene modified Schwann cells. RESULTS AND CONCLUSION： The staining of sciatic nerve and muscle fiber cross-sectional area was better in the experimental group than in the cell group and gene group （P 〈 0.01）, which was better in the celt and gene groups than the blank control group （P 〈 0.01）; but, there was no significant difference between the cell group and gene group （P 〉 0.05）. It indicated that neureotrophic factor-3 gene modified Schwann cells transplantation can reconstruct the microenvironment of nerve regeneration and promote repair of sciatic nerve defects.