维药买朱尼对关节软骨前炎性因子的影响

背景：骨关节炎病理过程中，白细胞介素1β被认为是促进软骨基质降解和关节软骨破坏的最重要的细胞因之一。目的：观察白细胞介素113在关节软骨中的表达，并观察维药买朱尼对其的影响。方法：将40只SD大鼠随机数字表法随机等分为模型对照组、维药买朱尼组、假手术组、正常对照组。模型对照组和维药买朱尼组采用改良Hulth造模法建立大鼠膝骨关节炎模型，假手术组仅显露膝关节，不切断韧带，不切除内侧半月板。维药买朱尼组建模第2周开始灌胃维药买朱尼10．31mg／（kg·d），模型组及假手术组大鼠均灌服等量生理盐水，连续4周。结果与结论：模型组软骨退变程度明显重于维药买朱尼组，模型组软骨大体评分及Mankin评分均明显高于维药买朱尼组（P〈0．05），模型组软骨细胞白细胞介素1β的表达强度亦明显高于维药买朱尼组（P〈0．05）。与正常对照组比较，假手术组软骨大体评分、Mankin评分及软骨细胞白细胞介素1β差异无显著性意义（P〉0．05）。结果说明，维药买朱尼可以抑制关节软骨前炎性因子白细胞介素1β的表达。

Effect of Uygur Medicine Maizhuni on inflammatory factors in the articular cartilage following osteoarthritis

BACKGROUND： Interieukin-lβ is consideredas an important cytokine for improvement of cartilage matrix degradation and destruction of articular cartilage during the pathological process of osteoarthdtis.OBJECTIVE： To establish the expression of interleukin-1β in the articular cartilage of osteoarthritis rats and to study the effect of Uygur Medicine Maizhuni. METHODS： Forty healthy Sprague Dawley rats were randomly assigned into four groups： Maizhuni intervention group, model control group, shame operation group, and normal control group. Knee osteoarthritis models were established in the model control and Maizhuni intervention groups using modified Hulth method. In the sham operation group, only the knee was exposed with no ligament resection and medial meniscectomy. Rats in the Maizhuni intervention group were treated with Uygur Medicine Maizhuni （10.31 mg/（kg-d）） and those in the model control and sham operation groups were administrated with saline by gavage at week 2 after model establishment. The treatment was continued for 4 weeks. RESULTS AND CONCLUSION： The degree of cartilage degeneration in the model control group was higher than that in the Maizhuni intervention group as observed by naked eye. General score and Mankin score in the model group were significantly higher than those in the Maizhuni intervention group （P 〈 0.05）. There was significantly greater expression of interleukin-1β in the chondrocytes of the model control group （P 〈 0.05）. Sham operation group did not differ from the normal control group in general score, Mankin score and interleukin-113 expression in the chondrocytes （P 〉 0.05）. These findings indicate that Uyghur Medicine Maizhuni may protect the articular cartilage by inhibiting the expression of interleukin-1β.