以4种基因诱导产前诊断绒毛细胞建立诱导性多能干细胞

背景：诱导性多能干细胞因具有多能性特征,可以诱导分化为特定的细胞,包括神经细胞、造血细胞等。目的：建立产前诊断绒毛细胞来源的诱导性多能干细胞。方法：运用反转录病毒介导4种基因hOct4、hSox2、hc-Myc、hKlf4诱导产前诊断绒毛细胞,对建立的诱导性多能干细胞进行多能性、体内外分化能力、核型等鉴定。结果与结论：建立的诱导性多能干细胞能维持自我更新状态,在蛋白和mRNA水平上高表达全能性的标志基因,具有体内、外分化潜能;在体外长期培养能维持正常核型。说明4种全能性基因转入绒毛细胞可获得具有多能性的诱导性多能干细胞,这为胎儿的细胞自体移植治疗提供理想来源,为产前诊断疾病机制研究提供很好的细胞模型。

Generation of induced pluripotent stem cells from villus cells after induction by four kinds of genes during prenatal diagnosis

BACKGROUND： Due to pluripotent characteristics, induced pluripotent stem cells can be induced to differentiate into the specific cells, including nerve cells and hematopoietic cells.OBJECTIVE： To establish the induced pluripotent stem cells generated from villus cells during prenatal diagnosis.METHODS： The prenatal diagnosis villus cells were induced by retroviral vectors encoding hOct4, hSox2, hKlf4 and hc-Myc. The pluripotency, differentiation ability and karyotype of the induced pluripotent stem cells were detected.RESULTS AND CONCLUSION： The induced pluripotent stem cells maintained the self-renewal ability with high pluripotent marker gene and protein expression and had in vivo and in vitro differentiation potential. The induced pluripotent stem cells could maintain normal karyotype in vitro after long-term culture. The induced pluripotent stem cells obtained from the prenatal diagnosis villus cells provide an ideal source for autologous cell-replacement therapy in the later life of the fetus, and can be used as a perfect model for the research of mechanism underlying prenatally diagnosed diseases.