脂肪基质细胞诱导分化为胆碱能神经元细胞

背景：脂肪基质细胞具有多向分化潜能,在特定条件下,可分化为中胚层起源组织的细胞及内、外胚层起源组织的细胞,在一定条件下可分化为胆碱能神经元。目的：探讨脂肪基质细胞的体外培养及其向胆碱能神经元细胞分化的能力。方法：取2-4周龄的SD大鼠腹股沟皮下脂肪垫,体外分离培养并传代,神经诱导培养基诱导培养后,免疫细胞学检测CD29、CD31和CD44的表达,进行表型鉴定。结果与结论：脂肪基质细胞在体外培养过程中贴壁生长,增殖能力强,其表面标记物CD29、CD44表达阳性,CD31表达阴性。经神经诱导培养基诱导培养后,分化的细胞大部分呈典型的神经元样细胞形态,巢蛋白、神经元特异性烯醇化酶、微管联合蛋白2表达阳性。诱导分化后胆碱乙酰转移酶表达阳性。说明脂肪基质细胞可分化为神经元样细胞,分化后的细胞具有合成胆碱乙酰转移酶的功能。

Adipose-derived stromal cells differentiate into cholinergic neurons

BACKGROUND： Adipose-derived stromal cells have multilineage differentiation potential, and it can differentiate into mesodermal cells and endodermal and ectodermal cells. Under certain conditions, they can differentiate into cholinergic neurons.
OBJECTIVE： To explore in vitro culture of adipose-derived stromal cells and their ability to differentiate into cholinergic neurons.
METHODS： Subcutaneous inguinal fat pads from 2-4 week-old Sprague-Dawley rats were taken and then in vitro isolated, cultured and passaged. After induced and cultured with neural induction medium, CD29, CD31 and CD44 expressions was detected by immunocytochemical tests and the phenotypic identification was performed.
RESULTS AND CONCLUSION： During the in vitro culture, adipose-derived stromal cells adhered to culture flask wall, proliferated strongly, and they were positive for CD29 and CD44, but negative for CD31.After induced and cultured with neural induction medium, the differentiated cells showed a typical neuron-like cells morphology, and the expression of nestin, neuron-specific enolase and microtubule-associated protein 2 was positive. The expression of choline acetyltransferase was positive after induction and differentiation. It indicates that adipose-derived stromal cells can differentiate into neuron-like cells and the differentiated cells have the function of synthesizing choline acetyltransferase.