髓核细胞诱导骨髓间充质干细胞向髓核方向的分化

背景：国内外已有很多文献相继报道了用骨髓间充质干细胞-藻酸盐复合植入材料来修复大鼠退变的腰椎间盘，但尚缺少对此复合物与正常髓核细胞之间的各项生物学指标的比较。目的：在Transwell非接触共培养条件下，研究髓核细胞对骨髓间充质干细胞的诱导作用。方法：全骨髓法培养大鼠骨髓间充质干细胞并鉴定，取第3代骨髓间充质干细胞及髓核细胞复合藻酸盐接种于Transwell共培养系统中，上室接种骨髓间充质干细胞藻酸盐复合物，下室接种髓核细胞藻酸盐复合物，共培养7d后回收上层骨髓间充质干细胞。结果与结论：RT-PCR方法检测共培养组Ⅱ型胶原、Sox-9和多功能蛋白聚糖的mRNA表达均接近正常髓核细胞。说明共培养条件下髓核细胞能诱导骨髓间充质干细胞向髓核方向分化。

Induced differentiation of nucleus pulposus cells from bone marrow mesenchymal stem cells

BACKGROUND： There have been many reports addressing repair of degenerative lumbar intervertebral discs in rats using alginate compounded with bone marrow mesenchymal stem cells. However, there are few reports describing biological indices of this compounded materia/versus normal nucleus pulposus cells. OBJECTIVE： To investigate the inducing effects of normal nucleus pulposus cells on bone marrow mesenchymal stem cells under Transwell non-contact co-culture condition. METHODS： Rat bone marrow mesenchymal stem cells were cultured from the whole bone marrow and identified. Passage 3 bone marrow mesenchymal stem cells and nucleus pulposus cells compounded onto alginate were inoculated into a Transwell co-culture system. Bone marrow mesenchymal stem cells compounded onto alginate were inoculated onto the upper layer, and nucleus pulposus cells compounded onto alginate were inoculated onto the lower layer. After 7 days of co-culture, bone marrow mesenchymalstem cells were retrieved from the upper layer. RESULTS AND CONCLUSION： RT-PCR results showed that type 1I collagen, Sox-9 and versian mRNA expression in the bone marrow mesenchymal stem cells ＋ nucleus pulposus cells group was similar to that in the normal nucleus pulposus cells group. These findings suggest that after co-cultured with nucleus pulposus cells, bone marrow mesenchymal stem cells can be induced to differentiate into nucleus pulposus cells.