微载体旋转立体培养成人退变髓核细胞及鉴定

微载体培养系统中，种子细胞在培养皿或生物反应器内悬浮的微载体表面上生长，能够在一定时间内收获大量种子细胞，可将种子细胞一微载体复合体直接植入病损部位，而不用依靠支架系统。微载体可以为同源的单层细胞的生长扩增提供足够的空间，同时能节省大量昂贵的生长因子和培养液。利用微载体生物反应器培养系统还可以较容易的进行氧气浓度和物理化学环境的调控，并监控环境中的pH值、氧饱和度、营养成分等，以得到细胞增殖和分化所需的特定环境。在培养过程中可以定期取出少量细胞进行分析而不必浪费其他的细胞。

Adult degenerative nucleus pulpous cells cultured by a microcarrier-based stirred culture system

BACKGROUND： Rotating bioreactor is a microcarrier-based stirred culture system that is currently used more often. OBJECTIVE： To culture the adult degenerative nucleus pulposus cells in vitro by a microcarrier-based stirred culture system, and then to amplify and identify the cells. METHODS： Nucleus pulposus specimens taken from patients with disc degenerative diseases were randomly divided into monolayer culture group and micro-carrier culture group. The cell morphology was observed by hematoxylin-eosin staining and Gimsa staining, the microstructure was observed under anelectron microscope, cell viability was detected by MTT method RESULTS AND CONCLUSION： The morphology of degenerative nucleus pulpous cells was identified by inverted phase contrast microscope, and the regression was showed by a transmission electron microscope. The microcarrier cultured cells were observed by an inverted phase contrast microscope and scanning electron microscope showing stereoscopic shape. In the logarithmic growth phase, the growth activity of microcarrier culture cells was significantly higher than that of the cells in monolayer culture. Microcarrier-based stirred culture system can stabilize the cell phenotype, and significantly stimulate the cellular activity of nucleus pulposus cells in the logarithmic growth phase.