| 类金刚石／酪蛋白磷酸肽复合薄膜修饰钛合金表面成骨细胞的增殖 |
| |
| 引用本文： | 卜银忠,王琳,冉海琼,陈军麟,张俊彦,刘斌.类金刚石／酪蛋白磷酸肽复合薄膜修饰钛合金表面成骨细胞的增殖[J].中国临床康复,2013(8):1367-1371. |
| |
| 作者姓名： | 卜银忠 王琳 冉海琼 陈军麟 张俊彦 刘斌 |
| |
| 作者单位： | [1]兰州大学口腔医学院,甘肃省兰州市730000 [2]兰州大学第二医院口腔科,甘肃省兰州市730000 [3]中国科学院兰州化学物理研究所固体润滑国家重点实验室,甘肃省兰州市730000 |
| |
| 基金项目： | 国家自然科学基金项目（50823008）;中央高校基本科研业务费专项资金自由探索面上项目（Izujbky-2011-96）. |
| |
| 摘 要： | 背景：钛合金表面沉积类金刚石薄膜可提高其摩擦和抗腐蚀性能，但缺乏生物活性，在其表面接枝生物蛋白分子是一种新的生物化学改性途径。目的：观察成骨细胞在类金刚石／酪蛋白磷酸肽复合薄膜修饰钛合金表面的增殖与黏附。方法：采用非平衡磁控溅射和多步组装方法在钛合金表面制备类金刚石，酪蛋白磷酸肽复合薄膜。将对数生长期的成骨细胞悬液接种于类金刚石／酪蛋白磷酸肽复合薄膜修饰的钛合金与纯钛合金试件上。结果与结论：类金刚石／酪蛋白磷酸肽复合薄膜修饰钛合金组细胞增殖率和黏附数量高于纯钛合金组（P〈0．05）。扫描电镜显示，类金刚石，酪蛋白磷酸肽复合薄膜修饰钛合金组成骨细胞胞体显著增大，表面粗糙，边界模糊不清，细胞呈充分的铺展状态；纯钛合金组成骨细胞胞体光滑，边界线条锐利清晰，伸展不良。表明类金刚石，酪蛋白磷酸肽复合薄膜可促进钛合金表面成骨细胞的增殖与黏附。
|
| 关 键 词： | 生物材料 组织工程骨材料 组织工程口腔材料 类金刚石薄膜 钛合金 酪蛋白磷酸肽 成骨细胞 增殖 黏附 国家自然科学基金 生物材料图片文章 |
Osteoblast proliferation on the surface of titanium alloy modified by diamond-like carbon/casein phosphopeptide composite film |
| |
| Bu Yin-zhong,Wang Lin,Ran Hai-qiong,Chen Jun-lin,Zhang Jun-yan,Liu Bin.Osteoblast proliferation on the surface of titanium alloy modified by diamond-like carbon/casein phosphopeptide composite film[J].Chinese Journal of Clinical Rehabilitation,2013(8):1367-1371. |
| |
| Authors: | Bu Yin-zhong Wang Lin Ran Hai-qiong Chen Jun-lin Zhang Jun-yan Liu Bin |
| |
| Institution: | 1 School of Stomatology, Lanzhou University, Lanzhou 730000, Gansu Province, China 2 Department of Stomatology, Lanzhou University Second Hospital, Lanzhou 730000, Gansu Province, China 3 National State Key Laboratory of Solid Lubrication, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, Gansu Province, China |
| |
| Abstract: | BACKGROUND： Titanium alloy surface modification with diamond-like carbon film can improve the frictionand corrosion resistance of titanium alloy, but it is lack of biological activity. So fixing biological protein molecules on the diamond-like carbon surface is a kind of new biological chemical modification method. OBJECTIVE： To observe the proliferation and adhesion of osteoblasts on the titanium alloy surface modification with diamond-like carbon/casein phosphopeptide composite film. METHODS： Using unbalanced magnetron sputtering and multi-step asSembly method diamond-like carbon/ casein phosphopeptide composite film was prepared on titanium alloy surface. The osteoblast suspension at logarithmic growth phase was seeded on the titanium alloy surface modified with or without the diamond-like carbon/casein phosphopeptide composite film. RESULTS AND CONCLUSION： Compared with the titanium alloy without modification, the adhesion and proliferation of osteoblasts were significantly increased after modification by diamond-like carbon/casein phosphopeptide composite film （P 〈 0.05）. The scanning electron microscope showed the osteoblast body on the surface of titanium alloy increased significantly, presenting with rough surface, fuzzy boundaries, and fully stretched after modification by diamond-like carbon/casein phosphopeptide composite film, but the osteoblasts on the surface of titanium alloy without modification displayed smooth surface, clear boundary, not fully stretched. It is indicated that the prepared diamond-like carbon/casein phosphopeptide composite film can significantly increase the biological activity of titanium alloy. |
| |
| Keywords: | biomaterials tissue engineering bone materials tissue engineering oral materials diamond-likecarbon film titanium alloy casein phosphopeptides osteoblasts proliferation adhesion the National NaturalScience Foundation of China biomaterial photographs-containing paper |
| 本文献已被 维普 等数据库收录！ |
|
