鹿脱蛋白松质骨的生物相容性

背景：经过脱蛋白去抗原处理的异种松质骨作为骨移植材料,具有天然的多孔结构、可塑性及一定的机械强度。目的：观察鹿脱蛋白松质骨的生物相容性。方法：①热源实验与急性毒性实验：将鹿新鲜骨、脱蛋白松质骨及深低温冻干脱蛋白松质骨浸提液分别注入家兔耳缘静脉与小鼠腹腔。②溶血实验：将兔血混悬液分别加入鹿新鲜骨、脱蛋白松质骨、深低温冻干脱蛋白松质骨、碳酸钠（阳性对照）、生理盐水（阴性对照）中。③凝血实验：将鹿新鲜骨、脱蛋白松质骨及深低温冻干脱蛋白松质骨分别加入兔正常混合血浆中。④肌袋实验：在小鼠大腿肌袋处分别植入新鲜鹿骨、鹿脱蛋白松质骨及深低温冻干脱蛋白松质骨。结果与结论：鹿脱蛋白松质骨及深低温冻干鹿脱蛋白松质骨无热源反应,未引起毒性、溶血及凝血反应,植入小鼠肌肉内后未发生排斥反应。新鲜鹿骨6种实验有轻度异常,但无动物死亡。表明鹿脱蛋白松质骨及深低温冻干鹿脱蛋白松质骨具有良好的生物相容性。

Biocompatibility of deer deproteinized cancellous bone

BACKGROUND： Antigen-extracted xenogeneic cancellous bone as a kind of bone implant material has a natural porous structure, plasticity, and certain mechanical strength.OBJECTIVE： To study the biocompatibility of the deer deproteinized cancellous bone. METHODS： Pyrogen test and acute toxicity test： Fresh deer bone, deproteinized cancellous bone, and free-dried deproteinized cancellous bone extracts were injected into the ear vein of rabbits and the abdominal cavity of mice. Hemolysis test： Rabbit mixed blood was added into the fresh deer bone, deproteinized cancellous bone, and free-dried deproteinized cancellous bone extracts, sodium carbonate （positive control）, and normal saline （negative control）. Coagulation test： Fresh deer bone, deproteinized cancellous bone, and free-dried deproteinized cancellous bone were placed into normal rabbit mixed blood. Long-term muscle test： Fresh deer bone, deproteinized cancellous bone, and free-dried deproteinized cancellous bone were implanted into the thigh muscle pouches of mice.RESULTS AND CONCLUSION： Deer deproteinized cancellous bone and free-dried deproteinized cancellous bone had no pyrogen reaction, toxicity, hemolysis and hemopexis reactions, and did not result in rejection reaction after implantation to the thigh muscle pouches of mice. There was a mild abnormality for the fresh deer bone in pyrogen, coagulation, hemolysis and long-term muscle tests without death. These findings indicate that deer deproteinized cancellous bone and free-dried deproteinized cancellous bone have good biocompatibility.