Expression of fibrinolytic and coagulation factors in cocultured human endothelial and smooth muscle cells |
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Authors: | Helenius Gisela Heydarkhan-Hagvall Sepideh Siegbahn Agneta Risberg Bo |
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Affiliation: | Department of Surgery, Wallenberg Laboratory for Cardiovascular Research, Sahlgrenska University Hospital, G?teborg University, Se-413 45 G?teborg, Sweden. |
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Abstract: | Interactions between endothelial cells and smooth muscle cells are interesting from a tissue-engineering point of view. We have developed a coculture system that allows direct contact between these two cell types. The fibrinolytic factors PAI-1, tPA, and uPA and the coagulation factor TF, were studied at the gene level by RT-PCR and at the protein level by ELISA. Significant changes of all studied factors were seen at the gene level in cocultured endothelial cells. tPA and TF were upregulated 4- and 7-fold, respectively, and PAI-1 and uPA were downregulated 4- and 1.5-fold, respectively, compared with single-cultured controls. In cocultured smooth muscle cells alterations of PAI-1 and TF were significant, with a 1.5-fold upregulation of PAI-1 and a 2.5-fold downregulation of TF. Results at the protein level mirrored the gene expression results. These findings indicate that cocultured endothelial cells are rendered both hypercoagulative and hyperfibrinolytic. |
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