大鼠坐骨神经夹伤后SSeCKS的表达变化

为了探讨大鼠坐骨神经夹伤后SSeCKS(Src抑制的蛋白激酶C底物)在神经中的表达变化及其意义,本研究制备了成年SD大鼠坐骨神经夹伤模型,通过Western blotting和免疫组织化学方法检测坐骨神经夹伤后SSeCKS表达的时空变化。Western blotting结果显示:大鼠坐骨神经夹伤后,SSeCKS的表达迅速升高,伤后6h即达到高峰,之后逐渐下降。免疫组织化学染色结果表明SSeCKS在神经夹伤两端高表达,以近端明显,呈簇状聚集。夹伤远端表达较近端稍低。远离夹伤处及相应对侧,SSeCKS的表达水平有所下降且分布较为均一。免疫荧光组织化学双标记结果显示:夹伤两端SSeCKS与S100和NF200的共存不明显,但可见部分SSeCKS与GAP43双标纤维。本研究提示大鼠坐骨神经夹伤可引起SSeCKS的表达变化,该变化可能参与周围神经损伤后某些伤害性刺激信号分子的转导并与损伤后神经的再生及功能修复有关。

EXPRESSION CHANGES OF SSeCKS IN THE CRUSHED RAT SCIATIC NERVE

In order to investigate the expression changes of SSeCKS in the crushed sciatic nerve, we made the sciatic nerve crush model of SD (Sprague-Dawley) rat and tested the changes of SSeCKS by Western blotting and immunohistochemical methods. The results of Western blotting showed that after injury, SSeCKS increased rapidly and reached its peak at 6 h, then it began to decrease. The results of immunohistochemical staining showed that expression of SSeCKS was higher in both sides of the crush site and was obviously in proximal part. It was in a cluster expression pattern. While in the distal part of the crush site, the expression level of SSeCKS was a little lower than that in the proximal part. In more distant region and the corresponding contrapart, the level of SSeCKS was lower and the distribution was uniform. The results of immunofluorescence double staining showed that SSeCKS did not have striking colocalization with S100 and NF200 in both proximal and distal sides of the crush site, but SSeCKS partly colocalized with GAP43. All results suggest that crush of rat sciatic nerve can cause the expression changes of SSeCKS, and it may be involved in transduction of some noxious stimulation signal molecules after the injury and also related with the nerve regeneration and function repair.